Cloned (Comment) | Organism |
---|---|
cloned into the pET-28a expression vector and transformed into Escherichia coli | Rhodococcus jostii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus jostii | Q0SCE7 | - |
- |
Purification (Comment) | Organism |
---|---|
partially purified by (NH4)2SO4 precipitation but it losts activity during attempted chromatographic steps | Rhodococcus jostii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
vanillin + NAD+ + H2O | vdh mutant Rhodococcus jostii RHA045 is unable to grow on 1 mM vanillin as the sole organic substrate but grows on 1 mM vanillate with kinetics similar to that of RHA1, mutant rescue by vdh harbouring pTipvdh plasmid | Rhodococcus jostii | vanillate + NADH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ro02986 | - |
Rhodococcus jostii |
vanillin dehydrogenase | - |
Rhodococcus jostii |
VDH | - |
Rhodococcus jostii |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme is involved in lignin degradation because one degradation product of lignin is vanillin | Rhodococcus jostii |