Literature summary for 1.2.3.1 extracted from

Paragas, E.M.; Humphreys, S.C.; Min, J.; Joswig-Jones, C.A.; Jones, J.P.
The two faces of aldehyde oxidase Oxidative and reductive transformations of 5-nitroquinoline (2017), Biochem. Pharmacol., 145, 210-217 .

Search for more literature for 1.2.3.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
allopurinol	does not affect the extent of the oxidation reactions of 5-nitroquinoline but instead increases the amount of reductive metabolite produced by around 60%	Homo sapiens
hydralazine	inhibits all three reactions of 5-nitroquinoline	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.073	-	5-nitroquinoline	metabolite 2-oxo-5-aminoquinoline, pH 7.4, 37°C	Homo sapiens
0.1	-	5-nitroquinoline	metabolite 5-aminoquinoline, presence of N-(2-(dimethylamino)ethyl)acridine-4-carboxamide, pH 7.4, 37°C	Homo sapiens
0.912	-	5-nitroquinoline	metabolite 2-oxo-5-nitroquinoline, pH 7.4, 37°C	Homo sapiens
0.912	-	5-nitroquinoline	metabolite 2-oxo-5-nitroquinoline, presence of N-(2-(dimethylamino)ethyl)acridine-4-carboxamide, pH 7.4, 37°C	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q06278	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-nitroquinoline + H2O + O2	-	Homo sapiens	2-oxo-5-nitroquinoline + 5-aminoquinoline + 2-oxo-5-aminoquinoline	-	?
N-(2-(dimethylamino)ethyl)acridine-4-carboxamide + H2O + O2	-	Homo sapiens	N-[(2-dimethylamino)ethyl] acridine-4-carboxamide-9(10H)-acridone + H2O2	-	?
phthalazine + H2O + O2	-	Homo sapiens	1-phthalazinone + H2O2	-	?

Synonyms

Synonyms	Comment	Organism
AOX1	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.005	-	5-nitroquinoline	metabolite 2-oxo-5-aminoquinoline, pH 7.4, 37°C	Homo sapiens
0.133	-	5-nitroquinoline	metabolite 2-oxo-5-nitroquinoline, pH 7.4, 37°C	Homo sapiens
0.18	-	5-nitroquinoline	metabolite 5-aminoquinoline, presence of N-(2-(dimethylamino)ethyl)acridine-4-carboxamide, pH 7.4, 37°C	Homo sapiens
1.31	-	5-nitroquinoline	metabolite 2-oxo-5-nitroquinoline, presence of N-(2-(dimethylamino)ethyl)acridine-4-carboxamide, pH 7.4, 37°C	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	in vitro reaction of 5-nitroquinoline with AOX under aerobic conditions generates the oxidized 2-oxo-5-nitroquinoline, the reduced 5-aminoquinoline and the oxidized/reduced 2-oxo-5-aminoquinoline metabolites. In human liver cytosol, coincubation of 5-nitroquinoline and AOX oxidative substrates N-(2-(dimethylamino)ethyl)acridine-4-carboxamide and phthalazine significantly increases the yield of the reduced metabolite, while oxidized metabolites production are lowered	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.07	-	5-nitroquinoline	metabolite 2-oxo-5-aminoquinoline, pH 7.4, 37°C	Homo sapiens
0.15	-	5-nitroquinoline	metabolite 2-oxo-5-nitroquinoline, pH 7.4, 37°C	Homo sapiens
0.17	-	5-nitroquinoline	metabolite 5-aminoquinoline, presence of N-(2-(dimethylamino)ethyl)acridine-4-carboxamide, pH 7.4, 37°C	Homo sapiens

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Release 2023.1 (January 2023)