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Literature summary for 1.2.3.4 extracted from

  • Goodwin, J.; Rana, H.; Ndungu, J.; Chakrabarti, G.; Moomaw, E.
    Hydrogen peroxide inhibition of bicupin oxalate oxidase (2017), PLoS ONE, 12, e0177164 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.3.4

Application

Application Comment Organism
analysis membrane inlet mass spectrometry assay to directly measure initial rates of carbon dioxide formation and oxygen consumption in the presence and absence of hydrogen peroxide Gelatoporia subvermispora

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Gelatoporia subvermispora

Inhibitors

Inhibitors Comment Organism Structure
H2O2 hydrogen peroxide is both a reversible noncompetitive inhibitor of the OxOx catalyzed oxidation of oxalate and an irreversible inactivator. The build-up of the turnover-generated hydrogen peroxide product leads to the inactivation of the enzyme. The introduction of catalase to reaction mixtures protects the enzyme from inactivation allowing reactions to proceed to completion. No changes in global protein structure take place in the presence of hydrogen peroxide Gelatoporia subvermispora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 oxalate pH 4, 25°C Gelatoporia subvermispora
0.47
-
 oxalate presence of 2 mM H2O2, pH 4, 25°C Gelatoporia subvermispora
0.56
-
 oxalate presence of 4 mM H2O2, pH 4, 25°C Gelatoporia subvermispora
0.76
-
 oxalate presence of 10 mM H2O2, pH 4, 25°C Gelatoporia subvermispora

Organism

Organism UniProt Comment Textmining
Gelatoporia subvermispora
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mesoxalate + O2 + 2 H+
-
 Gelatoporia subvermispora CO2 + oxalate + H2O2
-
 ?
oxalate + O2 + 2 H+
-
 Gelatoporia subvermispora 2 CO2 + 2 H2O2
-
 ?

Synonyms

Synonyms Comment Organism
OxOx
-
 Gelatoporia subvermispora