Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dihydroxyacetone phosphate | 10 mM, 70% inhibition | Caldicellulosiruptor bescii | |
additional information | bifunctional phosphoglycerate kinase/triose-phosphate isomerase is a strong inhibitor of GOR | Caldicellulosiruptor bescii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0389 | - |
oxidized ferredoxin | pH 8.0, 70°C | Caldicellulosiruptor bescii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Tungsten | large subunit GOR-L contains a single tungstopterin site and one iron-sulfur [4Fe-4S] cluster | Caldicellulosiruptor bescii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
gel filtration | Caldicellulosiruptor bescii |
315000 | - |
gel filtration | Caldicellulosiruptor bescii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldicellulosiruptor bescii | B9MQI2 and B9MQI1 | B9MQI2 i.e. large subunit GOR-L, B9MQI1 i.e. 4Fe-4S ferredoxin iron-sulfur binding domain protein, GOR-S | - |
Caldicellulosiruptor bescii DSM 6725 | B9MQI2 and B9MQI1 | B9MQI2 i.e. large subunit GOR-L, B9MQI1 i.e. 4Fe-4S ferredoxin iron-sulfur binding domain protein, GOR-S | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
48.3 | - |
substrate glyceraldehyde 3-phosphate, pH 8.0, 70°C | Caldicellulosiruptor bescii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyceraldehyde + H2O + oxidized ferredoxin | - |
Caldicellulosiruptor bescii | glycerate + H+ + reduced ferredoxin | - |
? | |
glyceraldehyde + H2O + oxidized ferredoxin | - |
Caldicellulosiruptor bescii DSM 6725 | glycerate + H+ + reduced ferredoxin | - |
? | |
glyceraldehyde 3-phosphate + H2O + oxidized ferredoxin | - |
Caldicellulosiruptor bescii | glycerate 3-phosphate + H+ + reduced ferredoxin | - |
? | |
glyceraldehyde 3-phosphate + H2O + oxidized ferredoxin | - |
Caldicellulosiruptor bescii DSM 6725 | glycerate 3-phosphate + H+ + reduced ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 60000, large subunit, 1 * 15000, small subunit, and tetramer of homodimers, | Caldicellulosiruptor bescii |
octamer | tetramer of homodimers, 4 * 60000, large subunit, 4 * 15000, small subunit, and dimer | Caldicellulosiruptor bescii |
Synonyms | Comment | Organism |
---|---|---|
Athe_0820 | gene name, 4Fe-4S ferredoxin iron-sulfur binding domain | Caldicellulosiruptor bescii |
Athe_0821 | - |
Caldicellulosiruptor bescii |
glyceraldehyde 3-phosphate oxidoreductase | - |
Caldicellulosiruptor bescii |
Gor | - |
Caldicellulosiruptor bescii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.57 | - |
oxidized ferredoxin | pH 8.0, 70°C | Caldicellulosiruptor bescii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]-center | large subunit GOR-L contains one iron-sulfur [4Fe-4S] cluster, small subunit GOR-S contains four [4Fe-4S] cluster | Caldicellulosiruptor bescii |
General Information | Comment | Organism |
---|---|---|
physiological function | deletion of either subunit results in a distinct growth phenotype on both C5 and C6 sugars, with an increased lag phase, but higher cell densities. GOR functions in parallel with the conventional glyceraldehyde-3-phosphate dehydrogenase | Caldicellulosiruptor bescii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47 | - |
glyceraldehyde 3-phosphate | pH 8, 70°C | Caldicellulosiruptor bescii |