Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-bipyridyl | - |
Thermococcus sp. | |
acetaldehyde | 5 mM, 50% inhibition | Thermococcus sp. | |
AsO2- | - |
Thermococcus sp. | |
CN- | relatively insensitive, 10% inhibition after 1 with 8 mM | Thermococcus sp. | |
iodoacetate | - |
Thermococcus sp. | |
Isovaleraldehyde | 4 mM, 50% inhibition | Thermococcus sp. | |
PCMB | - |
Thermococcus sp. | |
phenylacetaldehyde | 7 mM, 50% inhibition | Thermococcus sp. | |
ZnCl2 | - |
Thermococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
ES-1 ferredoxin | with crotonaldehyde as cosubstrate, at 85°C | Thermococcus sp. | |
0.016 | - |
acetaldehyde | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
0.028 | - |
Isovaleraldehyde | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
0.05 | - |
Indoleacetaldehyde | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
0.057 | - |
benzaldehyde | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
0.065 | - |
Salicylaldehyde | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
0.076 | - |
phenylacetaldehyde | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
0.13 | - |
crotonaldehyde | with benzyl viologen as cosubstrate, at 45°C | Thermococcus sp. | |
0.136 | - |
crotonaldehyde | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
0.15 | - |
propanal | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
0.169 | - |
benzyl viologen | with crotonaldehyde as cosubstrate, at 85°C | Thermococcus sp. | |
0.196 | - |
glyceraldehyde | with benzyl viologen as cosubstrate, at 45°C | Thermococcus sp. | |
1.422 | - |
methanal | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
1.8 | - |
acetate | with methylviologen as cosubstrate | Thermococcus sp. | |
2.9 | - |
methyl viologen | with crotonaldehyde as cosubstrate | Thermococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme contains 0.7 gatom of Ca per mol of enzyme | Thermococcus sp. | |
Iron | enzyme contains 3.6 gatom Fe per mol of enzyme | Thermococcus sp. | |
Iron | contains a single [4Fe-4S]+cluster with an S = 3/2 ground state | Thermococcus sp. | |
Mg2+ | enzyme contains 1.1 gatom of Mg per mol of enzyme | Thermococcus sp. | |
Tungsten | enzyme contains 0.9 gatom of tungsten per mol of enzyme | Thermococcus sp. | |
Zn2+ | the enzyme contains 0.2 gatom of Zn per mol of enzyme | Thermococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
2 * 67000, SDS-PAGE | Thermococcus sp. |
105000 | - |
gel filtration | Thermococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermococcus sp. | the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation | ? | - |
? | |
additional information | Thermococcus sp. ES-1 | the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation | ? | - |
? | |
additional information | Thermococcus sp. ES1 | the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus sp. | - |
- |
- |
Thermococcus sp. ES-1 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
extremely sensitive to inactivation by O2. The extent of inactivation is greatly dependent on the temperature of incubation | Thermococcus sp. |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:ethanol-grown cell | - |
Thermococcus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
221 | - |
- |
Thermococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Acetaldehyde + benzyl viologen | r | Thermococcus sp. | acetate + reduced benzyl viologen | - |
? | |
Acetaldehyde + benzyl viologen | r | Thermococcus sp. ES-1 | acetate + reduced benzyl viologen | - |
? | |
Acetaldehyde + benzyl viologen | r | Thermococcus sp. ES1 | acetate + reduced benzyl viologen | - |
? | |
Benzaldehyde + benzyl viologen | - |
Thermococcus sp. | Benzoate + reduced benzyl viologen | - |
? | |
Benzaldehyde + benzyl viologen | - |
Thermococcus sp. ES-1 | Benzoate + reduced benzyl viologen | - |
? | |
Benzaldehyde + benzyl viologen | - |
Thermococcus sp. ES1 | Benzoate + reduced benzyl viologen | - |
? | |
Crotonaldehyde + benzyl viologen | at less than 1% of the activity with benzyl viologen | Thermococcus sp. | E-2-Butenoate + reduced benzyl viologen | - |
? | |
Crotonaldehyde + ES-1 ferredoxin | - |
Thermococcus sp. | Crotonate + reduced ES-1 ferredoxin | - |
? | |
Crotonaldehyde + methyl viologen | - |
Thermococcus sp. | Crotonate + reduced methyl viologen | - |
? | |
Glyceraldehyde + benzyl viologen | - |
Thermococcus sp. | Glycerate + reduced benzyl viologen | - |
? | |
Indoleacetaldehyde + benzyl viologen | - |
Thermococcus sp. | Indoleacetate + reduced benzyl viologen | - |
? | |
Isovaleraldehyde + benzyl viologen | - |
Thermococcus sp. | Isovalerate + reduced benzyl viologen | - |
? | |
Isovaleraldehyde + benzyl viologen | - |
Thermococcus sp. ES-1 | Isovalerate + reduced benzyl viologen | - |
? | |
Isovaleraldehyde + benzyl viologen | - |
Thermococcus sp. ES1 | Isovalerate + reduced benzyl viologen | - |
? | |
Methanal + benzyl viologen | - |
Thermococcus sp. | Formate + reduced benzyl viologen | - |
? | |
additional information | the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation | Thermococcus sp. | ? | - |
? | |
additional information | the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation | Thermococcus sp. ES-1 | ? | - |
? | |
additional information | the enzyme possibly functions to oxidize aldehydes generated during amino acid catabolism, it is also possible that the enzyme generates aldehydes from organic acids produced by fermentation | Thermococcus sp. ES1 | ? | - |
? | |
Phenylacetaldehyde + benzyl viologen | - |
Thermococcus sp. | Phenylacetate + reduced benzyl viologen | - |
? | |
Phenylacetaldehyde + benzyl viologen | - |
Thermococcus sp. ES-1 | Phenylacetate + reduced benzyl viologen | - |
? | |
Phenylacetaldehyde + benzyl viologen | - |
Thermococcus sp. ES1 | Phenylacetate + reduced benzyl viologen | - |
? | |
Propanal + benzyl viologen | - |
Thermococcus sp. | Propanoate + reduced benzyl viologen | - |
? | |
Salicylaldehyde + benzyl viologen | - |
Thermococcus sp. | Salicylate + reduced benzyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 67000, SDS-PAGE | Thermococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
enzyme concentration 2.0 mg/ml, 50% loss of activity after 5 min | Thermococcus sp. |
85 | - |
enzyme concentration 2.0 mg/ml, 50% loss of activity after 1 min | Thermococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
acetaldehyde | with methyl viologen as cosubstrate | Thermococcus sp. | |
10.5 | - |
glyceraldehyde | with benzyl viologen as cosubstrate, at 45°C | Thermococcus sp. | |
13 | - |
Salicylaldehyde | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
22 | - |
crotonaldehyde | with benzyl viologen as cosubstrate, at 45°C | Thermococcus sp. | |
55 | - |
indolecetaldehyde | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
62 | - |
methyl viologen | with crotonaldehyde as cosubstrate, at 85°C | Thermococcus sp. | |
250 | - |
benzyl viologen | with crotonaldehyde as cosubstrate, at 85°C | Thermococcus sp. | |
269 | - |
crotonaldehyde | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
270 | - |
ES-1 ferredoxin | with crotonaldehyde as cosubstrate, at 85°C | Thermococcus sp. | |
272 | - |
Isovaleraldehyde | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
343 | - |
acetaldehyde | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
720 | - |
benzaldehyde | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
950 | - |
methanal | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
960 | - |
phenylacetaldehyde | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. | |
1100 | - |
propanal | with benzyl viologen as cosubstrate, at 85°C | Thermococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
around, at 80 C, with benzyl viologen and crotonaldehyde as substrate | Thermococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
molybdopterin | enzyme contains molybdopterin | Thermococcus sp. |