BRENDA - Enzyme Database show
show all sequences of 1.3.1.119

Purification and characterization of chlorobenzene cis-dihydrodiol dehydrogenase from Xanthobacter flavus 14p1

Spiess, E.; Goerisch, H.; Arch. Microbiol. 165, 201-205 (1996)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
8-hydroxyquinoline
1 mM, 54% inhibition
Xanthobacter flavus
HgCl2
0.1 mM, complete loss of activity, most potent inhibitor
Xanthobacter flavus
KCN
1 mM, 26% inhibition
Xanthobacter flavus
additional information
no inhibition by EDTA, 2,2-dipyridyl, and 1,10-phenanthroline
Xanthobacter flavus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
NAD+
pH 8.0, 30°C, cosubstrate: 3,6-dichlorobenzene cis-dihydrodiol
Xanthobacter flavus
0.02
-
NAD+
pH 8.0, 30°C, cosubstrate: benzene cis-dihydrodiol
Xanthobacter flavus
0.21
-
3,6-dichlorobenzene cis-dihydrodiol
pH 8.0, 30°C
Xanthobacter flavus
0.78
-
benzene cis-dihydrodiol
pH 8.0, 30°C
Xanthobacter flavus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
110000
-
gel filtration
Xanthobacter flavus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Xanthobacter flavus
-
-
-
Xanthobacter flavus DSM 10330
-
-
-
Purification (Commentary)
Commentary
Organism
-
Xanthobacter flavus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
32
-
substrate: 3,6-dichlorobenzene cis-dihydrodiol, pH 8.0, 30°C
Xanthobacter flavus
63
-
substrate: benzene cis-dihydrodiol, pH 8.0, 30°C
Xanthobacter flavus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,4-dichlorobenzene cis-dihydrodiol + NAD+
activity is 30% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus
3,4-dichlorocatechol + NADH + H+
-
-
-
?
3,4-dichlorobenzene cis-dihydrodiol + NAD+
activity is 30% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus DSM 10330
3,4-dichlorocatechol + NADH + H+
-
-
-
?
3,5-dichlorobenzene cis-dihydrodiol + NAD+
activity is 195% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus
3,5-dichlorocatechol + NADH + H+
-
-
-
?
3,5-dichlorobenzene cis-dihydrodiol + NAD+
activity is 195% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus DSM 10330
3,5-dichlorocatechol + NADH + H+
-
-
-
?
3,6-dichlorobenzene cis-dihydrodiol + NAD+
activity is 60% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus
3,6-dichlorocatechol + NADH + H+
-
-
-
?
3,6-dichlorobenzene cis-dihydrodiol + NAD+
activity is 60% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus DSM 10330
3,6-dichlorocatechol + NADH + H+
-
-
-
?
3,6-dimethylbenzene cis-dihydrodiol + NAD+
activity is 105% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus
3,6-dimethylcatechol + NADH + H+
-
-
-
?
3,6-dimethylbenzene cis-dihydrodiol + NAD+
activity is 105% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus DSM 10330
3,6-dimethylcatechol + NADH + H+
-
-
-
?
3-chlorobenzene cis-dihydrodiol + NAD+
activity is 170% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus
3-chlorocatechol + NADH + H+
-
-
-
?
3-chlorobenzene cis-dihydrodiol + NAD+
activity is 170% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus DSM 10330
3-chlorocatechol + NADH + H+
-
-
-
?
3-fluorobenzene cis-dihydrodiol + NAD+
activity is 145% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus
3-fluorocatechol + NADH + H+
-
-
-
?
3-methylbenzene cis-dihydrodiol + NAD+
activity is 185% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus
3-methylcatechol + NADH + H+
-
-
-
?
benzene cis-dihydrodiol + NAD+
-
746932
Xanthobacter flavus
catechol + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 26500, SDS-PAGE
Xanthobacter flavus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
14.5
-
3,6-dichlorobenzene cis-dihydrodiol
pH 8.0, 30°C
Xanthobacter flavus
14.5
-
NAD+
pH 8.0, 30°C, cosubstrate: 3,6-dichlorobenzene cis-dihydrodiol
Xanthobacter flavus
27.8
-
benzene cis-dihydrodiol
pH 8.0, 30°C
Xanthobacter flavus
27.8
-
NAD+
pH 8.0, 30°C, cosubstrate: benzene cis-dihydrodiol
Xanthobacter flavus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
-
optimum stability at 4°C
Xanthobacter flavus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
the enzyme accepts also NADP+ as cosubstrate. The activity with NADP+ is reduced to 14% of that with NAD+
Xanthobacter flavus
NADP+
the activity with NADP+ is reduced to 14% of that with NAD+
Xanthobacter flavus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Xanthobacter flavus
-
-
5.4
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
the enzyme accepts also NADP+ as cosubstrate. The activity with NADP+ is reduced to 14% of that with NAD+
Xanthobacter flavus
NADP+
the activity with NADP+ is reduced to 14% of that with NAD+
Xanthobacter flavus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
8-hydroxyquinoline
1 mM, 54% inhibition
Xanthobacter flavus
HgCl2
0.1 mM, complete loss of activity, most potent inhibitor
Xanthobacter flavus
KCN
1 mM, 26% inhibition
Xanthobacter flavus
additional information
no inhibition by EDTA, 2,2-dipyridyl, and 1,10-phenanthroline
Xanthobacter flavus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
NAD+
pH 8.0, 30°C, cosubstrate: 3,6-dichlorobenzene cis-dihydrodiol
Xanthobacter flavus
0.02
-
NAD+
pH 8.0, 30°C, cosubstrate: benzene cis-dihydrodiol
Xanthobacter flavus
0.21
-
3,6-dichlorobenzene cis-dihydrodiol
pH 8.0, 30°C
Xanthobacter flavus
0.78
-
benzene cis-dihydrodiol
pH 8.0, 30°C
Xanthobacter flavus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
110000
-
gel filtration
Xanthobacter flavus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Xanthobacter flavus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
32
-
substrate: 3,6-dichlorobenzene cis-dihydrodiol, pH 8.0, 30°C
Xanthobacter flavus
63
-
substrate: benzene cis-dihydrodiol, pH 8.0, 30°C
Xanthobacter flavus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,4-dichlorobenzene cis-dihydrodiol + NAD+
activity is 30% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus
3,4-dichlorocatechol + NADH + H+
-
-
-
?
3,4-dichlorobenzene cis-dihydrodiol + NAD+
activity is 30% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus DSM 10330
3,4-dichlorocatechol + NADH + H+
-
-
-
?
3,5-dichlorobenzene cis-dihydrodiol + NAD+
activity is 195% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus
3,5-dichlorocatechol + NADH + H+
-
-
-
?
3,5-dichlorobenzene cis-dihydrodiol + NAD+
activity is 195% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus DSM 10330
3,5-dichlorocatechol + NADH + H+
-
-
-
?
3,6-dichlorobenzene cis-dihydrodiol + NAD+
activity is 60% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus
3,6-dichlorocatechol + NADH + H+
-
-
-
?
3,6-dichlorobenzene cis-dihydrodiol + NAD+
activity is 60% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus DSM 10330
3,6-dichlorocatechol + NADH + H+
-
-
-
?
3,6-dimethylbenzene cis-dihydrodiol + NAD+
activity is 105% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus
3,6-dimethylcatechol + NADH + H+
-
-
-
?
3,6-dimethylbenzene cis-dihydrodiol + NAD+
activity is 105% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus DSM 10330
3,6-dimethylcatechol + NADH + H+
-
-
-
?
3-chlorobenzene cis-dihydrodiol + NAD+
activity is 170% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus
3-chlorocatechol + NADH + H+
-
-
-
?
3-chlorobenzene cis-dihydrodiol + NAD+
activity is 170% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus DSM 10330
3-chlorocatechol + NADH + H+
-
-
-
?
3-fluorobenzene cis-dihydrodiol + NAD+
activity is 145% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus
3-fluorocatechol + NADH + H+
-
-
-
?
3-methylbenzene cis-dihydrodiol + NAD+
activity is 185% compared to the activity with benzene cis-dihydrodiol
746932
Xanthobacter flavus
3-methylcatechol + NADH + H+
-
-
-
?
benzene cis-dihydrodiol + NAD+
-
746932
Xanthobacter flavus
catechol + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 26500, SDS-PAGE
Xanthobacter flavus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
14.5
-
3,6-dichlorobenzene cis-dihydrodiol
pH 8.0, 30°C
Xanthobacter flavus
14.5
-
NAD+
pH 8.0, 30°C, cosubstrate: 3,6-dichlorobenzene cis-dihydrodiol
Xanthobacter flavus
27.8
-
benzene cis-dihydrodiol
pH 8.0, 30°C
Xanthobacter flavus
27.8
-
NAD+
pH 8.0, 30°C, cosubstrate: benzene cis-dihydrodiol
Xanthobacter flavus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
-
optimum stability at 4°C
Xanthobacter flavus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Xanthobacter flavus
-
-
5.4
Other publictions for EC 1.3.1.119
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748609
Kunze
Degradation of chloroaromatic ...
Pseudomonas putida GJ31
Microbiology
155
4069-4083
2009
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2
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1
1
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748077
Pollmann
Chloromethylmuconolactones as ...
Cupriavidus sp. PS12
J. Bacteriol.
187
2332-2340
2005
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1
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3
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2
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1
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2
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1
1
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748076
Pollmann
Metabolism of dichloromethylc ...
Cupriavidus sp. PS12
J. Bacteriol.
184
5261-5274
2002
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1
1
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746812
Pollmann
Transformation of chlorinated ...
Cupriavidus sp. PS12
Appl. Environ. Microbiol.
67
4057-4063
2001
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-
1
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3
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746811
Raschke
cis-chlorobenzene dihydrodiol ...
Pseudomonas sp. P51
Appl. Environ. Microbiol.
65
5242-5246
1999
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1
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3
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9
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1
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9
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746933
Sommer
Enzymology of the degradation ...
Xanthobacter flavus, Xanthobacter flavus DSM 10330
Arch. Microbiol.
167
384-391
1997
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746932
Spiess
Purification and characteriza ...
Xanthobacter flavus, Xanthobacter flavus DSM 10330
Arch. Microbiol.
165
201-205
1996
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4
4
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1
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4
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1
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2
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13
1
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4
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1
2
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1
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4
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1
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1
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748143
Werlen
The broad substrate chloroben ...
Pseudomonas sp. P51
J. Biol. Chem.
271
4009-4016
1996
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