General Stability | Organism |
---|---|
dithiothreitol stabilizes | Escherichia coli |
EDTA stabilizes | Escherichia coli |
stability in Tris-HCl buffer higher than in phosphate buffer | Escherichia coli |
tyrosine stabilizes | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-tyrosine | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
NAD+ | - |
Escherichia coli | |
0.35 | 0.37 | prephenate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | 100000 | chorismate mutase-prephenate dehydrogenase bifunctional enzyme, gel filtration | Escherichia coli |
78000 | 100000 | chorismate mutase-prephenate dehydrogenase bifunctional enzyme, gel electrophoresis | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | Escherichia coli | biosynthesis of L-tyrosine | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12 | - |
Purification (Comment) | Organism |
---|---|
chorismate mutase-prephenate dehydrogenase bifunctional enzyme | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 0.1 M Tris-HCl buffer, pH 7.5, 1 mM EDTA, 1 mM DTT, 1 mM tyrosine, 70% w/v ammonium sulfate, several weeks | Escherichia coli |
4°C, 0.1 M phosphate buffer pH 7.6, over-night, complete loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | - |
Escherichia coli | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
prephenate + NAD+ | biosynthesis of L-tyrosine | Escherichia coli | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chorismate mutase-prephenate dehydrogenase bifunctional enzyme | complex with EC 5.4.99.5 | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Escherichia coli |