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Literature summary for 1.3.1.13 extracted from

  • Shlaifer, I.; Quashie, P.K.; Kim, H.Y.; Turnbull, J.L.
    Biochemical characterization of TyrA enzymes from Ignicoccus hospitalis and Haemophilus influenzae: A comparative study of the bifunctional and monofunctional dehydrogenase forms (2017), Biochim. Biophys. Acta, 1865, 312-320.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Ignicoccus hospitalis

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by L-Tyr Ignicoccus hospitalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.319
-
prephenate at pH 8.0 and 80┬░C Ignicoccus hospitalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40200
-
-
Ignicoccus hospitalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prephenate + NAD+ Ignicoccus hospitalis
-
4-hydroxyphenylpyruvate + CO2 + NADH
-
?
prephenate + NADP+ Ignicoccus hospitalis dual cofactor specificity with a preference for NADP+ 4-hydroxyphenylpyruvate + CO2 + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Ignicoccus hospitalis A8AAX2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Ignicoccus hospitalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prephenate + NAD+
-
Ignicoccus hospitalis 4-hydroxyphenylpyruvate + CO2 + NADH
-
?
prephenate + NADP+ dual cofactor specificity with a preference for NADP+ Ignicoccus hospitalis 4-hydroxyphenylpyruvate + CO2 + NADPH
-
?

Subunits

Subunits Comment Organism
? x * 40612, calculated from amino acid sequence Ignicoccus hospitalis

Synonyms

Synonyms Comment Organism
CM-PD bifunctional enzyme with chorismate mutase and prephenate dehydrogenase activities Ignicoccus hospitalis
tyrA
-
Ignicoccus hospitalis

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
54
-
melting temperature Ignicoccus hospitalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15
-
prephenate at pH 8.0 and 80┬░C Ignicoccus hospitalis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Ignicoccus hospitalis
NADP+ dual cofactor specificity with a preference for NADP+ Ignicoccus hospitalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
47
-
prephenate at pH 8.0 and 80┬░C Ignicoccus hospitalis