Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
to 1.85 A resolution. The overall structure displays the typical medium-chain dehydrogenase fold with two domains: the catalytic domain, residues 1-128 and 271-329, and the coenzyme-binding domain, residues 129-270, which are separated by a deep cleft that binds the cofactor NADP+ and the substrate | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y53F | substrate-pocket residue, large increase in Km values for all substrates and the cofactor, but mainly towards 3-buten-2-one and propenal with a 30fold increase | Homo sapiens |
Y59F | substrate-pocket residue, almost the same kinetic parameter values as the wild-type enzyme for 2-alkenals | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
NADPH | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.015 | - |
NADPH | mutant Y59F, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.035 | - |
3-buten-2-one | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.06 | - |
NADPH | mutant Y53F, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.13 | - |
4-hydroxy-2-hexenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.48 | - |
2-nonenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.55 | - |
4-hydroxy-2-nonenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.26 | - |
4-hydroxy-2-hexenal | mutant Y59F, pH 7.5, temperature not specified in the publication | Homo sapiens | |
3.67 | - |
4-hydroxy-2-hexenal | mutant Y53F, pH 7.5, temperature not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q08257 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nonenal + NADPH + H+ | - |
Homo sapiens | nonanal + NADP+ | - |
ir | |
3-buten-2-one + NADPH + H+ | - |
Homo sapiens | butan-2-one + NADP+ | - |
? | |
4-hydroxy-2-hexenal + NADPH + H+ | - |
Homo sapiens | 4-hydroxyhexadecanal + NADP+ | - |
? | |
4-hydroxy-2-nonenal + NADPH + H+ | - |
Homo sapiens | 4-hydroxynonanal + NADP+ | - |
? | |
additional information | enzyme additionally catalyzes the reduction of quinones, reaction of EC 1.6.5.5. The enzyme shows a low level of activity with cinnamaldehyde and no activity with 2-cyclohexen-1-one and 15-ketoprostaglandin E2. No activity is detected with nonanal and 2-nonene | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
zeta-Crystallin | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.113 | - |
2-nonenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.167 | - |
3-buten-2-one | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.15 | - |
4-hydroxy-2-nonenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.33 | - |
4-hydroxy-2-hexenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
4-hydroxy-2-hexenal | mutant Y53F, pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.23 | - |
2-nonenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.67 | - |
4-hydroxy-2-hexenal | mutant Y59F, pH 7.5, temperature not specified in the publication | Homo sapiens | |
2.1 | - |
4-hydroxy-2-nonenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
4.8 | - |
3-buten-2-one | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
10.2 | - |
4-hydroxy-2-hexenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens |