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Literature summary for 1.3.1.29 extracted from

  • Allen, C.C.R.; Walker, C.E.; Sharma, N.D.; Kerley, N.A.; Boyd, D.R.; Dalton, H.
    Selectivity studies of the benzene cis-dihydrodiol dehydrogenase enzyme from Pseudomonas putida ML2 with Vicinal diol substrates (2002), Biocatal. Biotransform., 20, 257-264.
No PubMed abstract available
Search for more literature for 1.3.1.29

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-1,2-dihydronaphthalene-1,2-diol + NAD+ Pseudomonas putida
-
 naphthalene-1,2-diol + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida 8859
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by ion exchange chromatography and gel filtration Pseudomonas putida

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:naphthalene-grown cell
-
 Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
 purified enzyme, substrate (1R,2S)-cis-1,2-dihydronaphthalene-1,2-diol Pseudomonas putida
16
-
 purified enzyme, substrate (1S,2R)-1,2-dihydroxy-3-phenylcyclohexa-3,5-diene Pseudomonas putida
19
-
 purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-chlorocyclohexa-3,5-diene Pseudomonas putida
20
-
 purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-fluorocyclohexa-3,5-diene Pseudomonas putida
20.6
-
 purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-bromocyclohexa-3,5-diene Pseudomonas putida
21.4
-
 purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-iodocyclohexa-3,5-diene Pseudomonas putida
22
-
 purified enzyme, substrate (1S,2R)-1,2-dihydroxy-3-methylcyclohexa-3,5-diene Pseudomonas putida
26
-
 purified enzyme, substrate cis-1,2-dihydrobenzene-1,2-diol Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R,2S)-cis-1,2-dihydronaphthalene-1,2-diol + NAD+ best substrate, stereospecific for the (1R,2S)-enantiomer, no activity with the (1S,2R)-enantiomer Pseudomonas putida naphthalene-1,2-diol + NADH
-
 ?
(1S,2R)-1,2-dihydroxy-3-methylcyclohexa-3,5-diene + NAD+
-
 Pseudomonas putida ?
-
 ?
(1S,2R)-1,2-dihydroxy-3-phenylcyclohexa-3,5-diene + NAD+
-
 Pseudomonas putida ?
-
 ?
(1S,2S)-1,2-dihydroxy-3-bromocyclohexa-3,5-diene + NAD+ stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer Pseudomonas putida ?
-
 ?
(1S,2S)-1,2-dihydroxy-3-chlorocyclohexa-3,5-diene + NAD+ stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer Pseudomonas putida ?
-
 ?
(1S,2S)-1,2-dihydroxy-3-fluorocyclohexa-3,5-diene + NAD+ stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer Pseudomonas putida ?
-
 ?
(1S,2S)-1,2-dihydroxy-3-iodocyclohexa-3,5-diene + NAD+ stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer Pseudomonas putida ?
-
 ?
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
 Pseudomonas putida benzene-1,2-diol + NADH
-
 ?
cis-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 Pseudomonas putida naphthalene-1,2-diol + NADH
-
 ?
additional information substrate specificity and stereoselectivity, no oxidation of any vicinal alkene diol substrate Pseudomonas putida ?
-
 ?

Synonyms

Synonyms Comment Organism
naphthalene cis-dihydrodiol dehydrogenase
-
 Pseudomonas putida
ncd
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas putida