Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
cortisone | - |
Rattus norvegicus | |
0.0022 | - |
4-androstene-3,17-dione | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + NADPH | Rattus norvegicus | - |
5beta-androstan-3,17-dione + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein | - |
Rattus norvegicus | - |
cortisone 5beta-reductase and reduction of androstenedione (3-oxo-5beta-steroid:NADP+ DELTA4-oxidoreductase activity, EC 1.3.1.23) catalyzed by the same catalytic site of a single protein | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.034 | - |
purified enzyme, substrate cortisone | Rattus norvegicus |
0.0341 | - |
- |
Rattus norvegicus |
0.0354 | - |
purified enzyme, substrate androstenedione | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + NADPH | - |
Rattus norvegicus | 5beta-androstan-3,17-dione + NADP+ | - |
? | |
androst-4-en-3,17-dione + NADPH | - |
Rattus norvegicus | 5beta-androstan-3,17-dione + NADP+ | - |
? | |
cortisone + NADPH + H+ | - |
Rattus norvegicus | 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
60 s, 50% loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rattus norvegicus | |
NADPH | absolutely required | Rattus norvegicus |