Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5beta-dihydrotestosterone | product inhibition | Sus scrofa | |
iodoacetate | - |
Sus scrofa | |
N-ethylmaleimide | - |
Sus scrofa | |
NADP+ | product inhibition | Sus scrofa | |
p-chloromercuribenzenesulfonate | - |
Sus scrofa | |
p-chloromercuribenzoate | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.085 | - |
NADPH | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sus scrofa | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | 30000 | gel filtration | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Storage Stability | Organism |
---|---|
4°C, 72 h, 0.05 M Tris-maleate buffer, pH 7.4-8.4, stable | Sus scrofa |
4°C, pH 8, 32 days, 25% loss of activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aldosterone + NADPH | - |
Sus scrofa | ? | - |
? | |
additional information | enzyme reduces a variety of C19 and C21 steroids, highest activity with aldosterone | Sus scrofa | ? | - |
? | |
testosterone + NADPH + H+ | - |
Sus scrofa | (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
Tris-maleate buffer | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 8 | pH 5.7: about 50% of activity maximum, pH 8.0: about 70% of activity maximum | Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
4°C, 30 days, 25% loss of activity | Sus scrofa |