Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Achromobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter sp. | I3V5V5 | JA81 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-2-cyano-3-phenylprop-2-enoic acid + NADPH | conversion rate measured using HPLC after 48 h. Conversion: 83%, ee: racemic | Achromobacter sp. | 2-cyano-3-phenylpropanoic acid + NADP+ | - |
? | |
(2Z)-3-(4-chlorophenyl)-3-cyanoprop-2-enoic acid + NADPH | conversion rate measured using HPLC after 48 h. Conversion: 80%, ee: 94% (R) | Achromobacter sp. | (R)-3-(4-chlorophenyl)-3-cyano-propanoic acid + NADP+ | - |
? | |
(Z)-3-cyano-3-phenyl-propenoic acid + NADPH + H+ | highest enantiomeric excess of 98% ee is achieved at a glucose concentration of 1% (w/v) with a 47% conversion for an 18 h reaction. Concentration of 2-propanol also affects the activity and selectivity of the reaction, and a concentration of 5% (v/v) results in the best substrate conversion and ee value of product. Conversion rate measured using HPLC after 48 h. Conversion: 80%, ee: 98% (R) | Achromobacter sp. | (R)-3-cyano-3-phenylpropanoic acid + NADP+ | - |
? | |
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADPH | conversion rate measured using HPLC after 48 h. Conversion: 99%, ee: 99% (R) | Achromobacter sp. | (R)-N-phenyl-2-methylsuccinimide + NADP+ | - |
? | |
ethyl (2Z)-3-nitro-2-phenylprop-2-enoate + NADPH | conversion rate measured using HPLC after 48 h. Conversion: 40%, ee: 45% (R) | Achromobacter sp. | ethyl (2R)-3-nitro-2-phenylpropanoate + NADP+ | - |
? | |
[(1E)-1-nitroprop-1-en-2-yl]benzene + NADPH | conversion rate measured using HPLC after 48 h. Conversion: 87%, ee: 97% (S) | Achromobacter sp. | (S)-1-nitro-2-phenylpropane + NADP+ | - |
? | |
[(1E)-2-nitroprop-1-en-1-yl]benzene + NADPH | conversion rate measured using HPLC after 48 h. Conversion: 60%, ee: racemic | Achromobacter sp. | 1-phenyl-2-nitropropane + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Achr-OYE3 | - |
Achromobacter sp. |
enoate reductase | - |
Achromobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
conversion rate drops to 20% at pH 6.0 | Achromobacter sp. |
7 | - |
highest conversion rate 54%, pH optimum | Achromobacter sp. |
8 | - |
48% conversion rate | Achromobacter sp. |