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Literature summary for 1.3.1.69 extracted from

  • Martin, R.C.; Mok, M.C.; Shaw, G.; Mok, D.W.
    An enzyme mediating the conversion of zeatin to dihydrozeatin in Phaseolus embryos (1989), Plant Physiol., 90, 1630-1635.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
glycerol, 20%, stabilizes Phaseolus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
trans-zeatin high molecular weight isoenzyme Phaseolus vulgaris
0.1 0.23 trans-zeatin low molecular weight isoenzyme, instability of the isoenzyme after gel filtration may contibute to the variation between experiments Phaseolus vulgaris

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Phaseolus vulgaris
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
low molecular weight isoenzyme, gel filtration Phaseolus vulgaris
55000
-
high molecular weight isoenzyme, gel filtration Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans-zeatin + NADPH Phaseolus vulgaris
-
dihydrozeatin + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Phaseolus vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
embryo immature Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
additional information
-
-
Phaseolus vulgaris

Storage Stability

Storage Stability Organism
-20Ā°C, in 0.05 M phosphate buffer containing 5 mM DTT, 0.5 mM EDTA, 5 mM NADPH, 0.5 M KCl and 20% glycerol: loss of about 10% of activity after 3 days Phaseolus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with: ribosylzeatin, cis-zeatin, O-xylosylzeatin, N6-(DELTA2-isopentenyl)adenine, or N6-(DELTA2-isotentenyl)adenosine Phaseolus vulgaris ?
-
?
trans-zeatin + NADPH
-
Phaseolus vulgaris dihydrozeatin + NADP+
-
?
zeatin + NADPH
-
Phaseolus vulgaris dihydrozeatin + NADP+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Phaseolus vulgaris

Cofactor

Cofactor Comment Organism Structure
NADPH required, NADH cannot substitute for NADPH Phaseolus vulgaris