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Literature summary for 1.3.1.71 extracted from

  • Jarman, T.R.; Gunatilaka, A.A.L.; Widdowson, D.A.
    Biosynthesis of steroids and terpenoids. XI. 24-Methylene sterol 24(28)-reductase of Saccharomyces cerevisiae (1975), Bioorg. Chem., 4, 202-211.
No PubMed abstract available
Search for more literature for 1.3.1.71

General Stability

General Stability Organism
25% glycerol and cofactor NADPH or NADH at 1.3 mM stabilize the enzyme Saccharomyces cerevisiae
anaerobiosis leads to loss of 15% activity after 24 h Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Saccharomyces cerevisiae
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH Saccharomyces cerevisiae terminal step in ergosterol biosynthesis, pathway overview, presursor analysis ergosterol + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
0°C, after 24 h: completely stable in presence of NADPH or NADH, 42% remaining activity in presence of 10 mM GSH or 2-mercaptoethanol, 33% remaining activity without additives, after 48 h: 93% remianing activity in prsence of 25% glycerol, 37% with 10% glycerol, and 13% remaining activity without additives Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH preferred substrate Saccharomyces cerevisiae ergosterol + NADP+
-
 ?
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH terminal step in ergosterol biosynthesis, pathway overview, presursor analysis Saccharomyces cerevisiae ergosterol + NADP+
-
 ?
additional information ergosta-7,22,24(28)-trien-3beta-ol, episterol, and fecosterol are poor substrates, 24,25-dihydro-24-methylene lanosterol is no substrate Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
24-methylene sterol 24(28)-reductase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADH about 10fold less active than NADPH Saccharomyces cerevisiae
NADPH preferred cofactor Saccharomyces cerevisiae