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Literature summary for 1.3.1.75 extracted from

  • Harada, J.; Mizoguchi, T.; Nomura, K.; Tamiaki, H.
    Isolation and structural determination of C8-vinyl-bacteriochlorophyll d from the bciA and bchU double mutant of the green sulfur bacterium Chlorobaculum tepidum (2014), Photosynth. Res., 121, 13-23 .
    View publication on PubMed

Engineering

Protein Variants Comment Organism
additional information construction of mutant tepdA lacking enzymes BciA and BchU, that catalyze reduction of the C8-vinyl group and methylation at the C20 position of bacteriochlorophyll (BChl) c, respectively, in the green sulfur bacterium Chlorobaculum tepidum, the mutant accumula C8-vinyl-bacteriochlorophyllide d derivative, determined by NMR to be (31R)-8-vinyl-12-ethyl-(R[V,E])BChl d. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c. Reconstitution of self-aggregates of C8V-BChl species in vitro. Comparison of chlorophyllide a derivatives in tepdA strains lacking bciA and/or bciU, overview Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,8-divinyl bacteriochlorophyllide a + NADPH + H+ Chlorobaculum tepidum
-
bacteriochlorophyllide a + NADP+
-
?
3,8-divinyl bacteriochlorophyllide a + NADPH + H+ Chlorobaculum tepidum TLS
-
bacteriochlorophyllide a + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum Q8KDI7
-
-
Chlorobaculum tepidum TLS Q8KDI7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,8-divinyl bacteriochlorophyllide a + NADPH + H+
-
Chlorobaculum tepidum bacteriochlorophyllide a + NADP+
-
?
3,8-divinyl bacteriochlorophyllide a + NADPH + H+
-
Chlorobaculum tepidum TLS bacteriochlorophyllide a + NADP+
-
?

Synonyms

Synonyms Comment Organism
BciA
-
Chlorobaculum tepidum
C8V reductase
-
Chlorobaculum tepidum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Chlorobaculum tepidum

General Information

General Information Comment Organism
malfunction the bciA deletion mutant of Chlorobaculum tepidum accumulates C8V pigments. The bciA mutant possesses mainly (31R)-8-vinyl-12-ethyl-(R[V,E])BChl c as in chlorosomal self-aggregates in addition to minor (31R)-8-vinyl-12-methyl-(R[V,M]) and (31S)-8-vinyl-12-ethyl-(S[V,E])BChls c Chlorobaculum tepidum