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Literature summary for 1.3.1.84 extracted from

  • Teufel, R.; Kung, J.; Kockelkorn, D.; Alber, B.; Fuchs, G.
    3-Hydroxypropionyl-coenzyme A dehydratase and acryloyl-coenzyme A reductase, enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in the Sulfolobales (2009), J. Bacteriol., 191, 4572-4581.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: EDTA, Zn2+, other divalent cations Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
acryloyl-CoA pH 6.0, 65┬░C Sulfurisphaera tokodaii
0.036
-
NADPH pH 6.0, 65┬░C Sulfurisphaera tokodaii

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Sulfurisphaera tokodaii
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ presence of 0.8 mol Zn2+ per mol of monomer, addition of Zn2+ is not stimulatory Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38600
-
1 * 39000, SDS-PAGE, 1 * 38600, calculated Sulfurisphaera tokodaii
39000
-
1 * 39000, SDS-PAGE, 1 * 38600, calculated Sulfurisphaera tokodaii
40000
-
x * 40000, SDS-PAGE Metallosphaera sedula
43000
-
gel filtration Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula
-
-
-
Sulfurisphaera tokodaii Q975C8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
2.9
-
65┬░C Metallosphaera sedula
18.7
-
pH 6.0, 65┬░C Sulfurisphaera tokodaii

Storage Stability

Storage Stability Organism
-20┬░C, 30% glycerol, stable for months Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acryloyl-CoA + NADPH + H+
-
Sulfurisphaera tokodaii propanoyl-CoA + NADP+
-
?
acrylyl-CoA + NADPH + H+
-
Metallosphaera sedula propanoyl-CoA + NADP+
-
?
additional information no substrate: NADH, crotonyl-CoA Sulfurisphaera tokodaii ?
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Metallosphaera sedula
monomer 1 * 39000, SDS-PAGE, 1 * 38600, calculated Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
acryloyl-coenzyme A reductase
-
Metallosphaera sedula
acryloyl-coenzyme A reductase
-
Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13
-
NADPH pH 6.0, 65┬░C Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Sulfurisphaera tokodaii

pH Range

pH Minimum pH Maximum Comment Organism
7.5
-
half-maximal activity Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
NADPH no cofactor: NADH Sulfurisphaera tokodaii