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Literature summary for 1.3.1.84 extracted from

  • Liu, Z.; Liu, T.
    Production of acrylic acid and propionic acid by constructing a portion of the 3-hydroxypropionate/4-hydroxybutyrate cycle from Metallosphaera sedula in Escherichia coli (2016), J. Ind. Microbiol. Biotechnol., 43, 1659-1670 .
    View publication on PubMed

Application

Application Comment Organism
synthesis successful reconstitution a portion of the 3-hydroxypropionate/4-hydroxybutyrate cycle from Metallosphaera sedula in Escherichia coli to produce acrylic acid and propionic acid, involving the acryloyl-CoA reductase (ACR), overview Metallosphaera sedula

Protein Variants

Protein Variants Comment Organism
additional information to construct an acrylic acid-producing pathway in Escherichia coli strain MG1655DELTArecADELTAendA(DE3)/pZL42, heterologous expression of malonyl-CoA reductase (MCR), malonate semialdehyde reductase (MSR), 3-hydroxypropionyl-CoA synthetase (3HPCS), and 3-hydroxypropionyl-CoA dehydratase (3HPCD) Metallosphaera sedula is accompanied by recombinant overexpression of succinyl-CoA synthetase (SCS) from Escherichia coli. The engineered strain produces 13.28 mg/l of acrylic acid. To construct a propionic acid-producing pathway, the same five genes are expressed, with the addition of Metallosphaera sedula acryloyl-CoA reductase (ACR). The engineered strain produces 1430 mg/l of propionic acid, detailed overview Metallosphaera sedula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acrylyl-CoA + NADPH + H+ Metallosphaera sedula
-
propanoyl-CoA + NADP+
-
?
acrylyl-CoA + NADPH + H+ Metallosphaera sedula DSM 5348
-
propanoyl-CoA + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula A4YGN2
-
-
Metallosphaera sedula DSM 5348 A4YGN2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylyl-CoA + NADPH + H+
-
Metallosphaera sedula propanoyl-CoA + NADP+
-
?
acrylyl-CoA + NADPH + H+
-
Metallosphaera sedula DSM 5348 propanoyl-CoA + NADP+
-
?
additional information the purified proteins of malonyl-CoA reductase (MCR) and 3-hydroxypropionyl-CoA dehydratase (3HPCD) from Sulfolobus tokodaii, and the proteins of malonate semialdehyde reductase (MSR), 3-hydroxypropionyl-CoA synthetase (3HPCS), and acryloyl-CoA reductase (ACR) from Metallosphaera sedula are used to convert malonyl-CoA to propionyl-CoA in vitro. Malonyl-CoA is almost completely converted to propionyl-CoA even at 23┬░C, and no other acyl-CoA products are detected, indicating that this system is robust. The turnover rate of this pathway is 37/min at 37┬░C Metallosphaera sedula ?
-
?
additional information the purified proteins of malonyl-CoA reductase (MCR) and 3-hydroxypropionyl-CoA dehydratase (3HPCD) from Sulfolobus tokodaii, and the proteins of malonate semialdehyde reductase (MSR), 3-hydroxypropionyl-CoA synthetase (3HPCS), and acryloyl-CoA reductase (ACR) from Metallosphaera sedula are used to convert malonyl-CoA to propionyl-CoA in vitro. Malonyl-CoA is almost completely converted to propionyl-CoA even at 23┬░C, and no other acyl-CoA products are detected, indicating that this system is robust. The turnover rate of this pathway is 37/min at 37┬░C Metallosphaera sedula DSM 5348 ?
-
?

Synonyms

Synonyms Comment Organism
Acr
-
Metallosphaera sedula
acryloyl-CoA reductase
-
Metallosphaera sedula
Msed_1426
-
Metallosphaera sedula

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Metallosphaera sedula

General Information

General Information Comment Organism
metabolism the enzyme is part of the 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation cycle, pahway overview Metallosphaera sedula