Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
apoenzyme by the sitting drop vapor diffusion method using 100 mM sodium acetate, 100 mM MES pH 6.5 and 30% (w/v) polyethyleneglycol 400 or enzyme in complex with isoniazid, NAD+ or NADH by the hanging drop vapor diffusion method using 100 mM HEPES pH 7.5, 8-10% (w/v) 2-methyl-2,4-pentanediol, 50 mM sodium citrate pH 6.5 | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isoniazid | - |
Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+ | Mycobacterium tuberculosis | - |
an acyl-[acyl-carrier protein] + NAD+ | - |
? | |
a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+ | Mycobacterium tuberculosis H37Rv | - |
an acyl-[acyl-carrier protein] + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WGR1 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WGR1 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-chelated His-Trap column chromatography, and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+ | - |
Mycobacterium tuberculosis | an acyl-[acyl-carrier protein] + NAD+ | - |
? | |
a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+ | - |
Mycobacterium tuberculosis H37Rv | an acyl-[acyl-carrier protein] + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enoyl-ACP reductase | - |
Mycobacterium tuberculosis |
InhA | - |
Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
57.6 | - |
melting temperature | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Mycobacterium tuberculosis |