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Literature summary for 1.3.7.6 extracted from

  • Stiefelmaier, J.; Ledermann, B.; Sorg, M.; Banek, A.; Geib, D.; Ulber, R.; Frankenberg-Dinkel, N.
    Pink bacteria-Production of the pink chromophore phycoerythrobilin with Escherichia coli (2018), J. Biotechnol., 274, 47-53 .
    View publication on PubMed
Search for more literature for 1.3.7.6

Cloned(Commentary)

Cloned (Comment) Organism
gene pebS, recombinant expression in Escherichia coli strain BL21 (DE3) from plasmid pTDho1pebS, Phycoerythrobilin production in batch-operated bioreactors, phycoerythrobilin enrichment in the bioreactor foam Prochlorococcus phage P-SSM2

Protein Variants

Protein Variants Comment Organism
additional information development and evaluation of an improved method for high yield production and purification of phycoerythrobilin (PEB) in Escherichia coli via heterologous expression where the two required enzymes heme oxygenase and PEB synthase subsequently convert the substrate heme provided by the host cell. Experiments in shaking flasks result in the highest product yield of 0.680 mg PEB per g cell dry weight, by induction with 0.1 mM IPTG. Scale-up to batch-operated fermentation in a 2 L bioreactor reached product concentrations up to 5.02 mg PEB/l by adjustment of aeration, induction time, media composition and supplementation of precursors, separation of PEB from developed foam above the culture, detailed overview Prochlorococcus phage P-SSM2

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ in cofactor ferredoxin Prochlorococcus phage P-SSM2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biliverdin IXalpha + 2 reduced ferredoxin Prochlorococcus phage P-SSM2
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 (3Z)-phycoerythrobilin + 2 oxidized ferredoxin
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 ?

Organism

Organism UniProt Comment Textmining
Prochlorococcus phage P-SSM2 Q58MU6
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biliverdin IXalpha + 2 reduced ferredoxin
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 Prochlorococcus phage P-SSM2 (3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
 ?

Synonyms

Synonyms Comment Organism
FDBR
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 Prochlorococcus phage P-SSM2
ferredoxin-dependent bilin reductase
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 Prochlorococcus phage P-SSM2
PebS
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 Prochlorococcus phage P-SSM2
phycoerythrobilin synthase
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 Prochlorococcus phage P-SSM2

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Prochlorococcus phage P-SSM2

General Information

General Information Comment Organism
metabolism phycoerythrobilin (PEB) is an open-chain tetrapyrrole that is derived from heme. The biosynthesis of PEB is, outgoing from heme, mediated by two classes of enzymes: heme oxygenases (HOs) and ferredoxin-dependent bilin reductases (FDBRs). In the first step, HOs (EC: 1.14.99.3) catalyze the ring-opening reaction of the cyclic tetrapyrrole heme at the alpha-mesocarbon bridge, yielding the open-chain tetrapyrrole biliverdin IXalpha (BV), CO and free iron. The sequential reductive cleavage of heme to BV consumes three molecules of O2 and seven electrons. HOs are involved in iron acquisition, oxidative-stress response and pigment biosynthesis. In plants and prokaryotes reduced ferredoxin and ascorbate are able to provide the electrons for the reaction. In the second step, BV is further reduced to PEB by a class of enzymes called ferredoxin-dependent bilin reductases (FDBRs) Prochlorococcus phage P-SSM2
physiological function the enzyme is involved in the biosynthesis of phycoerythrobilin Prochlorococcus phage P-SSM2