Protein Variants | Comment | Organism |
---|---|---|
C251A | insoluble | Mycobacterium ulcerans |
C269A | auxiliary [4Fe-4S] cluster I mutant, capable of catalyzing the reductive cleavage of SAM to form dAdo but incapable of converting MftA to MftA* or MftA** | Mycobacterium ulcerans |
C269A/C323A | insoluble | Mycobacterium ulcerans |
C30A/C34A/C37A | insoluble | Mycobacterium ulcerans |
C30A/C37A | radical S-adenosylmethionine mutant, can neither cleave SAM nor modify MftA | Mycobacterium ulcerans |
C310A/C341A | auxiliary [4Fe-4S] cluster II mutant, capable of catalyzing the reductive cleavage of SAM to form dAdo, incapable of converting MftA to MftA* or MftA** | Mycobacterium ulcerans |
C323A | auxiliary [4Fe-4S] cluster I mutant, capable of catalyzing the reductive cleavage of SAM to form dAdo, incapable of converting MftA to MftA* or MftA** | Mycobacterium ulcerans |
additional information | systematical replacement of Cys residues by Ala. The RS KO could neither cleave SAM nor modify MftA, consistent with the successful knockout of the RS cluster. Activity assays for Aux I and Aux II KO's also provided insightful results. Both Aux I and Aux II KO's were capable of catalyzing the reductive cleavage of SAM to form dAdo (Figure 3A), suggesting that the RS cluster remained intact and in an active conformation in the mutated proteins. However, when assayed against MftA, both Aux I and Aux II KO's were incapable of converting MftA to MftA* or MftA** | Mycobacterium ulcerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium ulcerans | A0PM49 | cf. EC 4.1.99.6 | - |
Mycobacterium ulcerans Agy99 | A0PM49 | cf. EC 4.1.99.6 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
C-terminal [mycofactocin precursor peptide MftA]-glycyl-L-valyl-L-tyrosine + S-adenosyl-L-methionine | - |
Mycobacterium ulcerans | C-terminal [mycofactocin precursor peptide MftA]-glycyl-L-valyl-4-[2-aminoethenyl]phenol + CO2 + 5'-deoxyadenosine + L-methionine | - |
? | |
C-terminal [mycofactocin precursor peptide MftA]-glycyl-L-valyl-L-tyrosine + S-adenosyl-L-methionine | - |
Mycobacterium ulcerans Agy99 | C-terminal [mycofactocin precursor peptide MftA]-glycyl-L-valyl-4-[2-aminoethenyl]phenol + CO2 + 5'-deoxyadenosine + L-methionine | - |
? | |
additional information | enzyme additionally displays the activity of EC 4.1.99.26, 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one synthase, forming a 3-amino-5-[(4-hydroxyphenyl) methyl]-4,4-dimethyl-2-pyrrolidinone moiety , i.e. MftA* | Mycobacterium ulcerans | ? | - |
- |
|
additional information | enzyme additionally displays the activity of EC 4.1.99.26, 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one synthase, forming a 3-amino-5-[(4-hydroxyphenyl) methyl]-4,4-dimethyl-2-pyrrolidinone moiety , i.e. MftA* | Mycobacterium ulcerans Agy99 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
mftC | - |
Mycobacterium ulcerans |
mycofactocin maturase | - |
Mycobacterium ulcerans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | residues Cys30, 34, and 37 are the radical S-adenosylmethionine ligands | Mycobacterium ulcerans | |
[4Fe-4S]-center | MftC binds a radical S-adenosylmethionine [4Fe-4S] cluster and two auxiliary [4Fe-4S] clusters that are required for MftA modification. Presence of S-adenosylmethionine and MftA affects the environments of the radical S-adenosylmethionine and Aux I cluster whereas the Aux II cluster is unaffected by the substrates. All three cluster are required for cataylsis | Mycobacterium ulcerans |