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Literature summary for 1.3.8.3 extracted from

  • Vogt, M.; Schuehle, K.; Koelzer, S.; Peschke, P.; Chowdhury, N.; Kleinsorge, D.; Buckel, W.; Essen, L.; Heider, J.
    Structural and functional characterization of an electron transfer flavoprotein involved in toluene degradation in strictly anaerobic bacteria (2021), J. Bacteriol., 201, e00326-19 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene bbsG, recombinant overexpression of the enzyme in Escherichia coli Geobacter metallireducens
gene bbsG, recombinant overexpression of the enzyme in Escherichia coli Thauera aromatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.098
-
(R)-2-benzylsuccinyl-CoA forward reaction, recombinant enzyme, pH 6.4, 50┬░C Geobacter metallireducens
0.14
-
(R)-2-benzylsuccinyl-CoA forward reaction, recombinant enzyme, pH 6.4, 50┬░C Thauera aromatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Geobacter metallireducens
-
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Desulfobacula toluolica
-
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Aromatoleum aromaticum
-
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Thauera aromatica
-
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Geobacter metallireducens GS-15
-
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Geobacter metallireducens DSM 7210
-
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Geobacter metallireducens ATCC 53774
-
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Desulfobacula toluolica DSM 7467
-
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Aromatoleum aromaticum EbN1
-
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Desulfobacula toluolica Tol2
-
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
?

Organism

Organism UniProt Comment Textmining
Aromatoleum aromaticum Q5P690 Azoarcus sp. EbN1
-
Aromatoleum aromaticum EbN1 Q5P690 Azoarcus sp. EbN1
-
Desulfobacula toluolica K0NF66
-
-
Desulfobacula toluolica DSM 7467 K0NF66
-
-
Desulfobacula toluolica Tol2 K0NF66
-
-
Geobacter metallireducens Q39VG7
-
-
Geobacter metallireducens ATCC 53774 Q39VG7
-
-
Geobacter metallireducens DSM 7210 Q39VG7
-
-
Geobacter metallireducens GS-15 Q39VG7
-
-
Thauera aromatica Q9KJE8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
4.4
-
reverse reaction, recombinant enzyme, pH 6.4, 50┬░C Geobacter metallireducens
7
-
reverse reaction, recombinant enzyme, pH 6.4, 50┬░C Thauera aromatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
Geobacter metallireducens (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
Desulfobacula toluolica (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
Aromatoleum aromaticum (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
Thauera aromatica (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
Geobacter metallireducens GS-15 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
Geobacter metallireducens DSM 7210 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
Geobacter metallireducens ATCC 53774 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
Desulfobacula toluolica DSM 7467 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
Aromatoleum aromaticum EbN1 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
Desulfobacula toluolica Tol2 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
?

Subunits

Subunits Comment Organism
? x * 45580, recombinant enzyme, SDS-PAGE Thauera aromatica
? x * 46360, recombinant enzyme, SDS-PAGE Geobacter metallireducens

Synonyms

Synonyms Comment Organism
bbgS
-
Geobacter metallireducens
bbgS
-
Desulfobacula toluolica
BbsG
-
Geobacter metallireducens
BbsG
-
Desulfobacula toluolica
BbsG
-
Aromatoleum aromaticum
BbsG
-
Thauera aromatica

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
50
-
recombinant enzyme Geobacter metallireducens
50
-
recombinant enzyme Thauera aromatica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
recombinant enzyme Geobacter metallireducens
8
-
recombinant enzyme Thauera aromatica

Cofactor

Cofactor Comment Organism Structure
electron transferring flavoprotein ETF, structural and functional characterization of the electron transfer flavoprotein involved in toluene degradation in the strictly anaerobic bacteria, binding kinetics analysis, overview. Unrooted phylogenetic tree representing evolutionary branches of the ETFAB family proteins Thauera aromatica
electron transferring flavoprotein structural and functional characterization of the electron transfer flavoprotein involved in toluene degradation in the strictly anaerobic bacteria, DNA and amino acid sequence determination and analysis, UniProt IDs: K0NC26 (etfA1) and K0NCE4 (etfB1). Unrooted phylogenetic tree representing evolutionary branches of the ETFAB family proteins, overview Desulfobacula toluolica
electron transferring flavoprotein structural and functional characterization of the electron transfer flavoprotein involved in toluene degradation in the strictly anaerobic bacteria, DNA and amino acid sequence determination and analysis, UniProt IDs: Q39VG4 (etfA) and Q39VG5 (etfB), binding kinetics analysis, overview. ETF crystal structure determination and analysis. Unrooted phylogenetic tree representing evolutionary branches of the ETFAB family proteins Geobacter metallireducens
electron transferring flavoprotein structural and functional characterization of the electron transfer flavoprotein involved in toluene degradation in the strictly anaerobic bacteria, DNA and amino acid sequence determination and analysis, UniProt IDs: Q5NYM0 (etfA) and Q5NYL9 (etfB). Unrooted phylogenetic tree representing evolutionary branches of the ETFAB family proteins, overview Aromatoleum aromaticum
FAD
-
Desulfobacula toluolica
FAD
-
Aromatoleum aromaticum
FAD 0.92 mol/mol enzyme Thauera aromatica
FAD 0.99 mol/mol enzyme Geobacter metallireducens
additional information in contrast to ETF, reduction of BbsG with dithionite leads directly to the fully reduced form without transient semiquinone formation Geobacter metallireducens
additional information in contrast to ETF, reduction of BbsG with dithionite leads directly to the fully reduced form without transient semiquinone formation Thauera aromatica