Cloned (Comment) | Organism |
---|---|
expression of recombinant wild-type and K304E mutant enzyme in Escherichia coli | Homo sapiens |
expression of recombinant wild-type and K304E mutant enzyme in Escherichia coli | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
hexadecanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens | |
0.0033 | - |
dodecanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens | |
0.0035 | - |
octanoyl-CoA | - |
Sus scrofa | |
0.0052 | - |
tetradecanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens | |
0.0075 | - |
hexanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens | |
0.0077 | - |
decanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens | |
0.009 | - |
hexanoyl-CoA | - |
Sus scrofa | |
0.012 | - |
octanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens | |
0.09 | - |
butanoyl-CoA | - |
Sus scrofa | |
0.12 | - |
butanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
K304E point mutation leading to a higher susceptibility of the mutant enzyme for degradation is one of several mutations, which are responsible for medium chain acyl-CoA dehydrogenase deficiency | - |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and K304E mutant enzyme | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.5 | - |
recombinant K304E mutant enzyme, measured with ferricenium assay | Homo sapiens |
24.9 | - |
recombinant wild-type enzyme, measured with ferricenium assay | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanoyl-CoA + acceptor | - |
Homo sapiens | crotonyl-CoA + reduced acceptor | - |
? | |
butanoyl-CoA + acceptor | - |
Sus scrofa | crotonyl-CoA + reduced acceptor | - |
? | |
hexanoyl-CoA + ferricenium | - |
Sus scrofa | trans-hex-2-enoyl-CoA + ferrocenium | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
37 | biphasic inactivation, half-lives: 3 h, fast phase, and more than 700 h, slow phase, at 28°C and 2 h, fast phase, and approx. 230 h, slow phase, at 37°C for the wild-type enzyme, the corresponding values for the K304E mutant enzyme are 1.7 h, 100 h at 28°C and 1.25 h and 55 h at 37°C | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.667 | - |
octadecanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens | |
2.92 | - |
hexadecanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens | |
6.33 | - |
butanoyl-CoA | ferricenium assay | Sus scrofa | |
8.33 | - |
tetradecanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens | |
8.83 | - |
decanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens | |
9 | - |
butanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens | |
9.5 | - |
dodecanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens | |
12.7 | - |
hexanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens | |
16.2 | - |
octanoyl-CoA | K304E mutant enzyme, ferricenium assay | Homo sapiens | |
18.3 | - |
octanoyl-CoA | ferricenium assay | Sus scrofa | |
20.8 | - |
hexanoyl-CoA | ferricenium assay | Sus scrofa |