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Literature summary for 1.3.99.4 extracted from
- Characterization and engineering of 3-ketosteroid-Delta1-dehydrogenase and 3-ketosteroid-9alpha-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9alpha-hydroxy-4-androstene-3,17-dione through the catabolism of sterols (2014), Metab. Eng., 24, 181-191 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | inactivation of KstD isoforms 1-3 leads to synthesis of about 6.02g/l of 9alpha-hydroxy-4-androstene-3,17-dione from 15 g/l of phytosterols. The product is mixed with 1.55 g/l of 4-androstene-3,17-dione as a major byproduct. Overexpression of the oxygenase component of 3-ketosteroid-9alpha-hydroxylase kshA, results in a yield of 9alpha-hydroxy-4-androstene-3,17-dione of 7.33 g/l | Mycolicibacterium neoaurum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Mycolicibacterium neoaurum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
4-androstene-17beta-ol-3-one | pH 7.0, 30°C | Mycolicibacterium neoaurum |  |
| 0.025 | - |
4-androstene-3,17-dione | pH 7.0, 30°C | Mycolicibacterium neoaurum | |
| 0.025 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 7.0, 30°C | Mycolicibacterium neoaurum | |
| 0.035 | - |
5alpha-androstane-17beta-ol-3-one | pH 7.0, 30°C | Mycolicibacterium neoaurum | |
| 0.0379 | - |
5alpha-androstane-17beta-ol-3-one | pH 7.0, 30°C | Mycolicibacterium neoaurum | |
| 0.039 | - |
progesterone | pH 7.0, 30°C | Mycolicibacterium neoaurum | |
| 0.045 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 7.0, 30°C | Mycolicibacterium neoaurum | |
| 0.06 | - |
progesterone | pH 7.0, 30°C | Mycolicibacterium neoaurum | |
| 0.065 | - |
4-androstene-3,17-dione | pH 7.0, 30°C | Mycolicibacterium neoaurum | |
| 0.082 | - |
4-androstene-17beta-ol-3-one | pH 7.0, 30°C | Mycolicibacterium neoaurum | |
| 0.085 | - |
4-androstene-17beta-ol-3-one | pH 7.0, 30°C | Mycolicibacterium neoaurum | |
| 0.105 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 7.0, 30°C | Mycolicibacterium neoaurum | |
| 0.125 | - |
progesterone | pH 7.0, 30°C | Mycolicibacterium neoaurum | |
| 0.16 | - |
4-androstene-3,17-dione | pH 7.0, 30°C | Mycolicibacterium neoaurum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium neoaurum | C8CJG6 | isoform KstD1 | - |
| Mycolicibacterium neoaurum | W0NR13 | isoform KstD2 | - |
| Mycolicibacterium neoaurum | W0NUN5 | isoform KstD3 | - |
| Mycolicibacterium neoaurum ATCC 25795 | C8CJG6 | isoform KstD1 | - |
| Mycolicibacterium neoaurum ATCC 25795 | W0NR13 | isoform KstD2 | - |
| Mycolicibacterium neoaurum ATCC 25795 | W0NUN5 | isoform KstD3 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-androstene-17beta-ol-3-one + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium neoaurum | 1,4-androstadiene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 4-androstene-17beta-ol-3-one + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium neoaurum ATCC 25795 | 1,4-androstadiene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium neoaurum | 1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium neoaurum ATCC 25795 | 1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 5alpha-androstane-17beta-ol-3-one + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium neoaurum | 5alpha-androst-1-ene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 5alpha-androstane-17beta-ol-3-one + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium neoaurum ATCC 25795 | 5alpha-androst-1-ene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium neoaurum | 9alpha-hydroxyandrost-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium neoaurum ATCC 25795 | 9alpha-hydroxyandrost-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| progesterone + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium neoaurum | pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| progesterone + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium neoaurum ATCC 25795 | pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61000, calculated | Mycolicibacterium neoaurum |
| ? | x * 54300, calculated | Mycolicibacterium neoaurum |
| ? | x * 60100, calculated | Mycolicibacterium neoaurum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| KSTD1 | - |
Mycolicibacterium neoaurum |
| KSTD2 | - |
Mycolicibacterium neoaurum |
| KSTD3 | - |
Mycolicibacterium neoaurum |
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