Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | activates | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzyme, expression in Sf21 cells using a baculovirus expression system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
M370L | mutation does not abolish basal activity and does not abrogate the activation of the enzyme by L-Leu | Homo sapiens |
M415L | mutation does not abolish basal activity and does not abrogate the activation of the enzyme by L-Leu | Homo sapiens |
R443S | mutation abolishes basal activity and totally abrogates the activation of the enzyme by L-Leu, 1-10 mM, in absence of other effectors. When ADP, 0.025-0.1 mM, is present, L-Leu (0.3-6.0 mM) activates the mutant enzyme up to 2000%. The mutant enzyme is much less sensitive to ADP than the wild-type enzyme, however at 1 mM ADP the Vmax is comparable with that of wild-type enzyme GLUD1 GDH. KM-value for 2-oxoglutarate is similar to wild-type value | Homo sapiens |
S331T | mutation does not abolish basal activity and does not abrogate the activation of the enzyme by L-Leu | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | IC50 for wild-type GLUD1: 0.0122 mM, IC50 for mutant enzyme R443S: 0.0162 mM, IC50 for mutant enzyme M415L: 0.0147 mM, IC50 for mutant enzyme M370L: 0.0113 mM, IC50 for mutant enzyme S331T: 0.0108 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values for 2-oxoglutaratefor mutant enzyme R443S, recombinant wild-type enzyme and wild-type enzyme from human liver | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADPH + NH3 | - |
Homo sapiens | L-glutamate + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GLUD1 | - |
Homo sapiens |
housekeeping glutamate dehydrogenase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0108 | - |
IC50 for mutant enzyme S331T: 0.0108 mM | Homo sapiens | GTP | |
0.0113 | - |
IC50 for mutant enzyme M370L: 0.0113 mM | Homo sapiens | GTP | |
0.0122 | - |
IC50 for wild-type GLUD1: 0.0122 mM | Homo sapiens | GTP | |
0.0147 | - |
IC50 for mutant enzyme M415L: 0.0147 mM | Homo sapiens | GTP | |
0.0162 | - |
IC50 for mutant enzyme R443S: 0.0162 mM | Homo sapiens | GTP |