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Literature summary for 1.4.3.12 extracted from

  • Tokieda, T.; Niimura, T.; Takamura,F.; Yamaha, T.
    Purification and some properties of cyclohexylamine oxidase from a Pseudomonas sp. (1977), J. Biochem., 81, 851-858.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 38% inhibition at 10 mM Pseudomonas sp.
Cu2+ complete inhibition at 10 mM Pseudomonas sp.
Hg2+ complete inhibition at 10 mM Pseudomonas sp.
N-ethylmaleimide 27% inhibition at 0.05 mM Pseudomonas sp.
p-chloromercuribenzoate 91% inhibition at 0.0005 mM, partially reversed by glutathione Pseudomonas sp.
Quinacrine 45% inhibition at 2 mM, partially released by FAD Pseudomonas sp.
Zn2+ 48% inhibition at 10 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
Cyclohexylamine
-
Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclohexylamine + O2 + H2O Pseudomonas sp. highly specific for alicyclic primary amines, oxygen is the only electron acceptor cyclohexanone + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, fractionation and chromatography steps Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
29.6
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,3,4-tetrahydro-2-naphthylamine + O2 + H2O 23% of the activity compared to cyclohexylamine Pseudomonas sp. ? + NH3 + H2O2
-
?
1,2-cyclohexanediamine + O2 + H2O 18% of the activity compared to cyclohexylamine Pseudomonas sp. cyclohexaneamine + NH3 + H2O2
-
?
cycloheptylamine + O2 + H2O 42% of the activity compared to cyclohexylamine Pseudomonas sp. cycloheptanone + NH3 + H2O2
-
?
cyclohexylamine + O2 + H2O highly specific for alicyclic primary amines, oxygen is the only electron acceptor Pseudomonas sp. cyclohexanone + NH3 + H2O2
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
35
-
-
Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas sp.