Application | Comment | Organism |
---|---|---|
synthesis | deracemization of racemic beta-amino alcohols is conducted by cyclohexylamine oxidase-catalyzed enantioselective deamination and transaminase-catalyzed enantioselective amination to afford (S)-beta-amino alcohols in excellent conversion (78-94%) and enantiomeric excess (above 99%) | Arthrobacter sp. TYUT010-15 |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 | Arthrobacter sp. TYUT010-15 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
(1R,2S)-1-amino-2-indanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
0.7 | - |
(1S,2R)-1-amino-2-indanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
0.9 | - |
(1S,2S)-2-aminocyclopentanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
1 | - |
(1S,2S)-2-aminocyclohexanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
1.1 | - |
(1R,2R)-2-aminocyclopentanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
1.3 | - |
(1R,2R)-2-aminocyclohexanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. TYUT010-15 | A0A7G7Y0T8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arthrobacter sp. TYUT010-15 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R,2R)-2-aminocyclohexanol + O2 + H2O | the enzyme shows highly R-selective deamination activity toward beta-amino alcohols | Arthrobacter sp. TYUT010-15 | cyclohexanone + NH3 + H2O2 | - |
? | |
(1R,2R)-2-aminocyclopentanol + O2 + H2O | the enzyme shows highly R-selective deamination activity toward beta-amino alcohols | Arthrobacter sp. TYUT010-15 | cyclopentanone + NH3 + H2O2 | - |
? | |
(1R,2S)-1-amino-2-indanol + O2 + H2O | the enzyme shows highly R-selective deamination activity toward beta-amino alcohols | Arthrobacter sp. TYUT010-15 | ? + NH3 + H2O2 | - |
? | |
(1S,2R)-1-amino-2-indanol + O2 + H2O | the enzyme shows highly R-selective deamination activity toward beta-amino alcohols | Arthrobacter sp. TYUT010-15 | ? + NH3 + H2O2 | - |
? | |
(1S,2S)-2-aminocyclohexanol + O2 + H2O | the enzyme shows highly R-selective deamination activity toward beta-amino alcohols | Arthrobacter sp. TYUT010-15 | cyclohexanone + NH3 + H2O2 | - |
? | |
(1S,2S)-2-aminocyclopentanol + O2 + H2O | the enzyme shows highly R-selective deamination activity toward beta-amino alcohols | Arthrobacter sp. TYUT010-15 | cyclopentanone + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ArCHAO | - |
Arthrobacter sp. TYUT010-15 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Arthrobacter sp. TYUT010-15 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 20°C: about 70% of maximal activity, 50°C: about 60% of maximal activity | Arthrobacter sp. TYUT010-15 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 30 | pH 7.0, 24 h, 100 mM sodium phosphate buffer, more than 70% residual activity | Arthrobacter sp. TYUT010-15 |
50 | - |
pH 7.0, 24 h, 100 mM sodium phosphate buffer, 10% residual activity | Arthrobacter sp. TYUT010-15 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
(1S,2R)-1-amino-2-indanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
1.5 | - |
(1S,2S)-2-aminocyclohexanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
1.6 | - |
(1S,2S)-2-aminocyclopentanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
4.9 | - |
(1R,2S)-1-amino-2-indanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
5.6 | - |
(1R,2R)-2-aminocyclopentanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
5.8 | - |
(1R,2R)-2-aminocyclohexanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Arthrobacter sp. TYUT010-15 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pH 6.0: about 70% of maximal activity, pH 10.0: about 65% of maximal activity | Arthrobacter sp. TYUT010-15 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | 20°C, 24 h, more than 80% residual activity | Arthrobacter sp. TYUT010-15 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
(1S,2S)-2-aminocyclohexanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
1.7 | - |
(1S,2R)-1-amino-2-indanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
1.8 | - |
(1S,2S)-2-aminocyclopentanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
4.5 | - |
(1R,2R)-2-aminocyclohexanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
5.1 | - |
(1R,2R)-2-aminocyclopentanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 | |
8.2 | - |
(1R,2S)-1-amino-2-indanol | pH 7.0, 30°C | Arthrobacter sp. TYUT010-15 |