Protein Variants | Comment | Organism |
---|---|---|
H244A | binds substrate analogues with higher dissociation constants and presents lower kcat/Km values in the reduction of fumarate | Escherichia coli |
H244S | binds substrate analogues with higher dissociation constants and presents lower kcat/Km values in the reduction of fumarate | Escherichia coli |
H351A | binds substrate analogues with higher dissociation constants and presents lower kcat/Km values in the reduction of fumarate | Escherichia coli |
H351S | binds substrate analogues with higher dissociation constants and presents lower kcat/Km values in the reduction of fumarate | Escherichia coli |
R386L | binds substrate analogues with higher dissociation constants and presens lower kcat/Km values in the reduction of fumarate | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
fumarate reductase in anaerobic conditions | - |
Purification (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + H2O + O2 | fumarate or succinate can be electron acceptor instead of O2 | Escherichia coli | oxaloacetate + NH3 + H2O2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli |