Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Rhodotorula toruloides |
Protein Variants | Comment | Organism |
---|---|---|
M213E | mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme | Rhodotorula toruloides |
M213R | mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme | Rhodotorula toruloides |
Q339E | mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme | Rhodotorula toruloides |
Q339N | mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme | Rhodotorula toruloides |
R285A/D/K/Q | inactive | Rhodotorula toruloides |
S335G | mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme | Rhodotorula toruloides |
S335H | mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme | Rhodotorula toruloides |
S335R | mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme | Rhodotorula toruloides |
Y223F | mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme | Rhodotorula toruloides |
Y223S | mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme | Rhodotorula toruloides |
Y238F | mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme | Rhodotorula toruloides |
Y238R | mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme | Rhodotorula toruloides |
Y238S | mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme | Rhodotorula toruloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
D-alanine | mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.4 | - |
D-alanine | mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.4 | - |
D-alanine | mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.4 | - |
D-alanine | mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.5 | - |
D-alanine | mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.6 | - |
D-alanine | mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.7 | - |
D-alanine | mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.8 | - |
D-alanine | wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
1 | - |
cephalosporin C | mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
1 | - |
cephalosporin C | mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
1.1 | - |
D-alanine | mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
1.1 | - |
D-alanine | mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
1.3 | - |
D-arginine | mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
1.3 | - |
D-alanine | mutant enzyme Q339N, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
1.7 | - |
cephalosporin C | mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
1.9 | - |
cephalosporin C | mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
1.9 | - |
cephalosporin C | mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
2 | - |
D-Aspartate | mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
2 | 3 | D-arginine | mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
2.5 | - |
D-arginine | mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
2.5 | - |
cephalosporin C | mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
2.8 | - |
cephalosporin C | mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
3.8 | - |
cephalosporin C | mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
4.3 | - |
cephalosporin C | mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
5 | - |
cephalosporin C | wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
5.2 | - |
D-Aspartate | mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
5.5 | - |
D-arginine | mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
5.5 | - |
D-arginine | mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
6.7 | - |
D-arginine | mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
8.7 | - |
cephalosporin C | mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
9.2 | - |
D-arginine | mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
9.7 | - |
D-arginine | mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
13 | - |
cephalosporin C | mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
13 | - |
D-arginine | mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
15 | - |
D-Aspartate | mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
15 | - |
D-Aspartate | mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
17 | - |
D-Aspartate | mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
18 | - |
D-alanine | mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
18 | - |
D-Aspartate | wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
18 | - |
D-arginine | wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
28 | - |
cephalosporin C | mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
29 | - |
D-arginine | mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
30 | - |
D-Aspartate | mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
33 | - |
D-Aspartate | mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
40 | - |
D-alanine | mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
53 | - |
D-Aspartate | mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
61 | - |
D-Aspartate | mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
72 | - |
D-Aspartate | mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
98 | - |
D-Aspartate | mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
150 | - |
D-Aspartate | mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Rhodotorula toruloides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula toruloides | P80324 | - |
- |
Purification (Comment) | Organism |
---|---|
phenyl-Sepharose column chromatography | Rhodotorula toruloides |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
104 | - |
purified wild type enzyme, at 25°C | Rhodotorula toruloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O + O2 | - |
Rhodotorula toruloides | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
D-alanine + H2O + O2 | - |
Rhodotorula toruloides | pyruvate + NH3 + H2O2 | - |
? | |
D-arginine + H2O + O2 | - |
Rhodotorula toruloides | 5-guanidino-2-oxopentanoate + NH3 + H2O2 | - |
? | |
D-aspartate + H2O + O2 | - |
Rhodotorula toruloides | oxaloacetate + NH3 + H2O2 | - |
? | |
additional information | the wild-type DAAO is mainly active on neutral D-amino acids, while basic D-amino acids are poor substrates and the acidic ones are virtually not oxidized | Rhodotorula toruloides | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-amino acid oxidase | - |
Rhodotorula toruloides |
DAAO | - |
Rhodotorula toruloides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
D-Aspartate | mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.2 | - |
D-arginine | kcat less than 0.2/s, mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.26 | - |
D-Aspartate | mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.37 | - |
D-Aspartate | mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.43 | - |
D-arginine | mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.5 | - |
D-Aspartate | mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.5 | - |
D-Aspartate | wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.56 | - |
D-Aspartate | mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.63 | - |
D-alanine | mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.67 | - |
D-Aspartate | mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.79 | - |
D-alanine | mutant enzyme Q339N, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
0.9 | - |
D-Aspartate | mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
1 | - |
D-Aspartate | mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
1.3 | - |
cephalosporin C | mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
2.1 | - |
D-arginine | mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
2.5 | - |
D-Aspartate | mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
2.6 | - |
D-arginine | mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
3.2 | - |
D-Aspartate | mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
3.2 | - |
D-arginine | mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
3.6 | - |
cephalosporin C | mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
3.6 | - |
D-arginine | mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
3.7 | - |
D-alanine | mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
3.9 | - |
D-Aspartate | mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
4.4 | - |
cephalosporin C | mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
6 | - |
D-Aspartate | mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
6.5 | - |
D-arginine | mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
6.9 | - |
D-alanine | mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
7 | - |
cephalosporin C | mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
7.3 | - |
cephalosporin C | mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
7.3 | - |
D-arginine | mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
7.8 | - |
cephalosporin C | mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
8.6 | - |
D-arginine | mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
9.2 | - |
D-alanine | mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
10 | - |
D-alanine | mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
11 | - |
D-arginine | mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
14 | - |
D-arginine | mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
15 | - |
D-alanine | mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
15 | - |
cephalosporin C | mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
17 | - |
cephalosporin C | mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
20 | - |
D-arginine | wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
24 | - |
cephalosporin C | mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
24 | - |
cephalosporin C | mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
25 | - |
D-alanine | mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
25 | - |
D-alanine | mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
26 | - |
cephalosporin C | mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
48 | - |
D-alanine | mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
50 | - |
cephalosporin C | mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
67 | - |
D-alanine | mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
72 | - |
cephalosporin C | wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
75 | - |
D-alanine | mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides | |
81 | - |
D-alanine | wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value | Rhodotorula toruloides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Rhodotorula toruloides |