Protein Variants | Comment | Organism |
---|---|---|
D242V/Q253R/D304V | mutant with altered substrate specificity | Rhodotorula toruloides |
E220D/Y224G | decreased catalytic activity for D-Ala compared to the wild type enzyme | Sus scrofa |
E222D/Y224G | decreased catalytic activity for D-Ala compared to the wild type enzyme | Sus scrofa |
G281C | inactive | Homo sapiens |
G313A | decreased catalytic activity for D-Ala compared to the wild type enzyme | Sus scrofa |
H307L | Kd for FAD is 28fold higher with respect to the wild type enzyme while activity is mostly retained | Sus scrofa |
H324L | inactive | Trigonopsis variabilis |
H324N | decreased activity | Trigonopsis variabilis |
H324Q | decreased activity | Trigonopsis variabilis |
H324R | inactive | Trigonopsis variabilis |
L118H | mutant with altered substrate specificity | Rhodotorula toruloides |
M156L | mutant shows increased H2O2 resistance | Trigonopsis variabilis |
M209L | mutant shows increased H2O2 resistance | Trigonopsis variabilis |
Q144R | mutant with altered substrate specificity | Rhodotorula toruloides |
R221D/Y224G | decreased catalytic activity for D-Ala compared to the wild type enzyme | Sus scrofa |
Y223S | strongly decreased turnover number | Rhodotorula toruloides |
Y228F | 50% activity compared to the wild type enzyme, 120fold decreased reduction rate | Sus scrofa |
Y238F | the mutation decreases the rate of product release und to a lesser extend the rate of substrate binding and does not alter significantly the substrate specificity of the enzyme | Rhodotorula toruloides |
Y238S | the mutation decreases the rate of product release und to a lesser extend the rate of substrate binding and does not alter significantly the substrate specificity of the enzyme | Rhodotorula toruloides |
General Stability | Organism |
---|---|
immobilisation on PEI 25 kDa Sepabeads and treatment with 0.5% glutaraldehyde solution at pH 7-8 and 25°C for 16 h leads to 160fold increased enzyme stability | Trigonopsis variabilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzoate | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
O2 | at pH 8.3 and 25°C | Sus scrofa | |
0.8 | - |
O2 | at pH 8.3 and 25°C | Trigonopsis variabilis | |
1.2 | - |
O2 | at pH 8.3 and 25°C | Homo sapiens | |
2 | - |
D-alanine | at pH 8.3 and 25°C | Sus scrofa | |
2.3 | - |
O2 | at pH 8.3 and 25°C | Rhodotorula toruloides | |
2.6 | - |
D-alanine | at pH 8.3 and 25°C | Rhodotorula toruloides | |
4.5 | - |
D-alanine | at pH 8.3 and 25°C | Trigonopsis variabilis | |
8.8 | - |
D-alanine | at pH 8.3 and 25°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Trigonopsis variabilis | 5777 | - |
peroxisome | - |
Rhodotorula toruloides | 5777 | - |
peroxisome | - |
Sus scrofa | 5777 | - |
peroxisome | - |
Homo sapiens | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Rhodotorula toruloides |
40000 | - |
2 * 40000, SDS-PAGE | Homo sapiens |
80000 | - |
SDS-PAGE | Rhodotorula toruloides |
80000 | - |
SDS-PAGE | Sus scrofa |
80000 | - |
SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14920 | - |
- |
Rhodotorula toruloides | P80324 | - |
- |
Sus scrofa | P00371 | - |
- |
Trigonopsis variabilis | Q99042 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O + O2 | - |
Trigonopsis variabilis | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
cephalosporin C + H2O + O2 | - |
Rhodotorula toruloides | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
cephalosporin C + H2O + O2 | - |
Sus scrofa | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
cephalosporin C + H2O + O2 | - |
Homo sapiens | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
D-alanine + H2O + O2 | - |
Trigonopsis variabilis | pyruvate + NH3 + H2O2 | - |
? | |
D-alanine + H2O + O2 | - |
Rhodotorula toruloides | pyruvate + NH3 + H2O2 | - |
? | |
D-alanine + H2O + O2 | - |
Sus scrofa | pyruvate + NH3 + H2O2 | - |
? | |
D-alanine + H2O + O2 | - |
Homo sapiens | pyruvate + NH3 + H2O2 | - |
? | |
D-methionine + H2O + O2 | by far best substrate | Trigonopsis variabilis | 4-methylthio-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
D-proline + H2O + O2 | best substrate | Sus scrofa | 2-oxopentanoate + NH3 + H2O2 | - |
? | |
D-valine + H2O + O2 | best substrate | Rhodotorula toruloides | alpha-ketoisovaleric acid + NH3 + H2O2 | - |
? | |
additional information | best substrate is by far D-methionine followed by D-phenylalanine, D-tryptophan, D-valin, and D-alanine | Trigonopsis variabilis | ? | - |
? | |
additional information | best substrate is D-valine followed by D-tryptophan, D-phenylalanine, D-alanine, and D-cysteine | Rhodotorula toruloides | ? | - |
? | |
additional information | D-aspartate and D-glutamate are no substrates | Trigonopsis variabilis | ? | - |
? | |
additional information | D-aspartate and D-glutamate are no substrates | Rhodotorula toruloides | ? | - |
? | |
additional information | D-aspartate and D-glutamate are no substrates | Sus scrofa | ? | - |
? | |
additional information | D-aspartate and D-glutamate are no substrates | Homo sapiens | ? | - |
? | |
additional information | D-proline is the best substrate followed by D-methionine, D-alanine, D-norleucine, D-isoleucine, and D-phenylalanine | Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 40000, SDS-PAGE | Rhodotorula toruloides |
homodimer | 2 * 40000, SDS-PAGE | Homo sapiens |
homodimer | x-ray crystallography | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
D-amino acid oxidase | - |
Trigonopsis variabilis |
D-amino acid oxidase | - |
Rhodotorula toruloides |
D-amino acid oxidase | - |
Sus scrofa |
D-amino acid oxidase | - |
Homo sapiens |
DAAO | - |
Trigonopsis variabilis |
DAAO | - |
Rhodotorula toruloides |
DAAO | - |
Sus scrofa |
DAAO | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
stable up to 35°C, sharp decrease in activity at higher temperatures | Rhodotorula toruloides |
40 | - |
stable up to 40°C, the enzyme has decreased stability at temperatures beyond and is completely inactivated at 65°C | Trigonopsis variabilis |
55 | 70 | soluble enzyme remains stable up to 55°C and retains more than 70% activity after 60 incubation, thermal stability up to 70°C is improved by chemical modification with soluble dextran | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
D-alanine | at pH 8.3 and 25°C | Sus scrofa | |
14.7 | - |
D-alanine | at pH 8.3 and 25°C | Homo sapiens | |
53 | - |
D-alanine | at pH 8.3 and 25°C | Trigonopsis variabilis | |
350 | - |
D-alanine | at pH 8.3 and 25°C | Rhodotorula toruloides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.2 | - |
Rhodotorula toruloides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent | Trigonopsis variabilis | |
FAD | dependent | Rhodotorula toruloides | |
FAD | dependent | Sus scrofa | |
FAD | dependent | Homo sapiens |