Application | Comment | Organism |
---|---|---|
biotechnology | the enzyme is used as a biocatalyst in cephalosporin C conversion on industrial scale | Trigonopsis variabilis |
Cloned (Comment) | Organism |
---|---|
stable expression in Pichia pastoris with 7fold enhancement of the intracellular level of oxidase activity, expression optimization by codon redesign as well as efficient subcellular targeting of the enzyme to peroxisomes | Trigonopsis variabilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development of a robust and highly active Pichia pastoris TvDAO whole-cell biocatalyst in a multistep engineering process for use in cephalosporin C conversion on industrial scale, overview. Usage of a fed-batch cultivation of the multicopy strain | Trigonopsis variabilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-amino acid + H2O + O2 | Trigonopsis variabilis | - |
2-oxo acid + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trigonopsis variabilis | Q6R4Q9 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
intracellular activity of recombinant DAO in transformed Pichia pastoris, overview | Trigonopsis variabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-amino acid + H2O + O2 | - |
Trigonopsis variabilis | 2-oxo acid + NH3 + H2O2 | - |
? | |
D-amino acid + H2O + O2 | via formation of an imino acid | Trigonopsis variabilis | 2-oxo acid + NH3 + H2O2 | - |
? | |
additional information | conversion of cephalosporine C to 7-aminocephalosporanic acid and spontaneous decarboxylation of oxoadipyl-7-amino cephalosporanic acid is promoted by the H2O2 formed in the oxidase reaction of TvDAO | Trigonopsis variabilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAO | - |
Trigonopsis variabilis |