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Literature summary for 1.4.3.3 extracted from

  • Yasukawa, K.; Motojima, F.; Ono, A.; Asano, Y.
    Expansion of the substrate specificity of porcine kidney D-amino acid oxidase for S-stereoselective oxidation of 4-Cl-benzhydrylamine (2018), ChemCatChem, 10, 3500-3505 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.3

Protein Variants

Protein Variants Comment Organism
I230A/R283G mutant accepts 1-(4-chlorophenyl)-1-phenylmethanamine as substrate, activity with (R)-1-phenylethylamine is diminished 10fold as compared with the Y228L/R283G variant Sus scrofa
I230C/R283G accepts 1-(4-chlorophenyl)-1-phenylmethanamine as substrate Sus scrofa
I230F/R283G accepts 1-(4-chlorophenyl)-1-phenylmethanamine as substrate Sus scrofa
R283G mutant has completely lost the activity for D-amino acids but accepts 1-(4-chlorophenyl)-1-phenylmethanamine as substrate Sus scrofa
Y228L about 20% of wild-type activity wih phenylalanine Sus scrofa
Y228L/R283G mutant accepts 1-(4-chlorophenyl)-1-phenylmethanamine as substrate Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
 1,1-diphenylmethanamine mutant I230C/R283G, 30°C, pH 8.0 Sus scrofa
2.63
-
 1,1-diphenylmethanamine mutant I230F/R283G, 30°C, pH 8.0 Sus scrofa
2.94
-
 (RS)-1-(4-chlorophenyl)-1-phenylmethanamine mutant I230A/R283G, 30°C, pH 8.0 Sus scrofa
2.96
-
 (RS)-1-(4-chlorophenyl)-1-phenylmethanamine mutant I230C/R283G, 30°C, pH 8.0 Sus scrofa
3.73
-
 (RS)-1-(4-chlorophenyl)-1-phenylmethanamine mutant I230F/R283G, 30°C, pH 8.0 Sus scrofa
5.01
-
 D-phenylalanine wild-type, 30°C, pH 8.0 Sus scrofa
5.23
-
 1,1-diphenylmethanamine mutant I230A/R283G, 30°C, pH 8.0 Sus scrofa
6.25
-
 (1R)-1-phenylethan-1-amine mutant I230F/R283G, 30°C, pH 8.0 Sus scrofa
6.9
-
 (1R)-1-phenylethan-1-amine mutant I230C/R283G, 30°C, pH 8.0 Sus scrofa
6.98
-
 (1R)-1-phenylethan-1-amine mutant R283G, 30°C, pH 8.0 Sus scrofa
7.28
-
 (1R)-1-phenylethan-1-amine mutant I230A/R283G, 30°C, pH 8.0 Sus scrofa
7.95
-
 (1R)-1-phenylethan-1-amine mutant Y228L/R283G, 30°C, pH 8.0 Sus scrofa
8.41
-
 D-phenylalanine mutant Y228L, 30°C, pH 8.0 Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa P00371
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R)-1-phenylethan-1-amine + H2O + O2 no substrate of wild-type Sus scrofa 1-phenylethan-1-one + NH3 + H2O2
-
 ?
(RS)-1-(4-chlorophenyl)-1-phenylmethanamine + H2O + O2 no substrate of wild-type Sus scrofa (4-chlorophenyl)(phenyl)methanone + NH3 + H2O2
-
 ?
1,1-diphenylmethanamine + H2O + O2 no substrate of wild-type Sus scrofa diphenylmethanone + NH3 + H2O2
-
 ?
D-phenylalanine + H2O + O2
-
 Sus scrofa phenylpyruvic acid + NH3 + H2O2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.047
-
 (1R)-1-phenylethan-1-amine mutant I230F/R283G, 30°C, pH 8.0 Sus scrofa
0.111
-
 (1R)-1-phenylethan-1-amine mutant I230C/R283G, 30°C, pH 8.0 Sus scrofa
0.349
-
 (1R)-1-phenylethan-1-amine mutant I230A/R283G, 30°C, pH 8.0 Sus scrofa
0.366
-
 1,1-diphenylmethanamine mutant I230F/R283G, 30°C, pH 8.0 Sus scrofa
1.46
-
 (1R)-1-phenylethan-1-amine mutant R283G, 30°C, pH 8.0 Sus scrofa
1.72
-
 D-phenylalanine mutant Y228L, 30°C, pH 8.0 Sus scrofa
3.3
-
 1,1-diphenylmethanamine mutant I230A/R283G, 30°C, pH 8.0 Sus scrofa
3.42
-
 1,1-diphenylmethanamine mutant I230C/R283G, 30°C, pH 8.0 Sus scrofa
4.33
-
 (RS)-1-(4-chlorophenyl)-1-phenylmethanamine mutant I230F/R283G, 30°C, pH 8.0 Sus scrofa
5.19
-
 D-phenylalanine wild-type, 30°C, pH 8.0 Sus scrofa
5.48
-
 (RS)-1-(4-chlorophenyl)-1-phenylmethanamine mutant I230C/R283G, 30°C, pH 8.0 Sus scrofa
6.01
-
 (RS)-1-(4-chlorophenyl)-1-phenylmethanamine mutant I230A/R283G, 30°C, pH 8.0 Sus scrofa
9.93
-
 (1R)-1-phenylethan-1-amine mutant Y228L/R283G, 30°C, pH 8.0 Sus scrofa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.008
-
 (1R)-1-phenylethan-1-amine mutant I230F/R283G, 30°C, pH 8.0 Sus scrofa
0.016
-
 (1R)-1-phenylethan-1-amine mutant I230C/R283G, 30°C, pH 8.0 Sus scrofa
0.048
-
 (1R)-1-phenylethan-1-amine mutant I230A/R283G, 30°C, pH 8.0 Sus scrofa
0.141
-
 1,1-diphenylmethanamine mutant I230F/R283G, 30°C, pH 8.0 Sus scrofa
0.21
-
 (1R)-1-phenylethan-1-amine mutant R283G, 30°C, pH 8.0 Sus scrofa
0.215
-
 D-phenylalanine mutant Y228L, 30°C, pH 8.0 Sus scrofa
0.629
-
 1,1-diphenylmethanamine mutant I230A/R283G, 30°C, pH 8.0 Sus scrofa
1.04
-
 D-phenylalanine wild-type, 30°C, pH 8.0 Sus scrofa
1.15
-
 (RS)-1-(4-chlorophenyl)-1-phenylmethanamine mutant I230F/R283G, 30°C, pH 8.0 Sus scrofa
1.26
-
 (1R)-1-phenylethan-1-amine mutant Y228L/R283G, 30°C, pH 8.0 Sus scrofa
1.45
-
 1,1-diphenylmethanamine mutant I230C/R283G, 30°C, pH 8.0 Sus scrofa
1.85
-
 (RS)-1-(4-chlorophenyl)-1-phenylmethanamine mutant I230C/R283G, 30°C, pH 8.0 Sus scrofa
2.05
-
 (RS)-1-(4-chlorophenyl)-1-phenylmethanamine mutant I230A/R283G, 30°C, pH 8.0 Sus scrofa