Cloned (Comment) | Organism |
---|---|
- |
Leishmania major |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme null mutants lack 5-methyltetrahydrofolate and cannot utilize exogenous homocysteine for growth. In mouse model of infection, null mutants show good infectivity and virulence | Leishmania major |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: S-adenosyl-L-methionine, no substrate inhibition up to 0.25 mM | Leishmania major |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34800 | - |
x * 34800, calculated | Leishmania major |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
expression in Saccharomyces cerevisiae | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | protein sequence shows no obvious intracellular targeting sequences | Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyltetrahydrofolate + NAD+ | - |
Leishmania major | 5,10-methylenetetrahydrofolate + NADH | - |
? | |
5-methyltetrahydrofolate + NADP+ | - |
Leishmania major | 5,10-methylenetetrahydrofolate + NADPH | - |
? | |
5-methyltetrahydrofolate + oxidized menadione | - |
Leishmania major | 5,10-methylenetetrahydrofolate + reduced menadione | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34800, calculated | Leishmania major |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
52 | - |
NADH | phosphate buffer | Leishmania major | |
74 | - |
NADPH | phosphate buffer | Leishmania major | |
87 | - |
NADH | Tris buffer | Leishmania major | |
140 | - |
NADPH | Tris buffer | Leishmania major |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | dual-cofactor specificity with NADH and NADPH under physiological conditions | Leishmania major | |
NADPH | dual-cofactor specificity with NADH and NADPH under physiological conditions | Leishmania major |