Protein Variants | Comment | Organism |
---|---|---|
additional information | selection of cell line with altered enzyme due to decreased methotrexate-sensitivity | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methasquin | - |
Mus musculus | |
methotrexate | wild-type | Mus musculus | |
pyrimethamine | - |
Mus musculus | |
trimethoprim | folate antagonist drug | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
NADPH | altered enzyme | Mus musculus | |
0.003 | - |
7,8-dihydrofolate | wild-type enzyme | Mus musculus | |
0.01 | - |
7,8-dihydrofolate | altered enzyme | Mus musculus | |
0.03 | - |
NADPH | wild-type | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
altered enzyme encoded by amplified genes in cultured fibroblasts | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | cultured 3T6 mouse embryo fibroblasts | Mus musculus | - |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
1 mM NADPH protects against inactivation | Mus musculus |
55 | - |
half-life: 8 min | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.243 | - |
7,8-dihydrofolate | altered enzyme | Mus musculus | |
4.67 | - |
7,8-dihydrofolate | wild-type enzyme | Mus musculus |