Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R44A | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44C | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44D | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44E | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44F | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44G | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44I | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44K | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44L | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44M | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44N | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44P | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44Q | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44S | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44T | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44V | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44W | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
R44Y | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
7,8-dihydrofolate | mutant enzyme R44H, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0008 | - |
7,8-dihydrofolate | mutant enzyme R44V, at 15°C, pH not specified in the publication | Escherichia coli | |
0.001 | - |
7,8-dihydrofolate | mutant enzyme R44Y, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0011 | - |
7,8-dihydrofolate | mutant enzyme R44C, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0014 | - |
7,8-dihydrofolate | mutant enzyme R44L, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0015 | - |
7,8-dihydrofolate | mutant enzyme R44G, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0016 | - |
7,8-dihydrofolate | mutant enzyme R44A, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0016 | - |
7,8-dihydrofolate | wild type enzyme, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0019 | - |
7,8-dihydrofolate | mutant enzyme R44E, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0021 | - |
7,8-dihydrofolate | mutant enzyme R44I, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0023 | - |
7,8-dihydrofolate | mutant enzyme R44M, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0024 | - |
7,8-dihydrofolate | mutant enzyme R44Q, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0025 | - |
7,8-dihydrofolate | mutant enzyme R44K, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0025 | - |
7,8-dihydrofolate | mutant enzyme R44N, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0025 | - |
7,8-dihydrofolate | mutant enzyme R44T, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0027 | - |
7,8-dihydrofolate | mutant enzyme R44F, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0029 | - |
7,8-dihydrofolate | mutant enzyme R44P, at 15°C, pH not specified in the publication | Escherichia coli | |
0.003 | - |
7,8-dihydrofolate | mutant enzyme R44D, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0031 | - |
7,8-dihydrofolate | mutant enzyme R44S, at 15°C, pH not specified in the publication | Escherichia coli | |
0.0037 | - |
7,8-dihydrofolate | mutant enzyme R44W, at 15°C, pH not specified in the publication | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | Escherichia coli | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | - |
Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHFR | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
7,8-dihydrofolate | mutant enzyme R44P, at 15°C, pH not specified in the publication | Escherichia coli | |
0.69 | - |
7,8-dihydrofolate | mutant enzyme R44D, at 15°C, pH not specified in the publication | Escherichia coli | |
0.89 | - |
7,8-dihydrofolate | mutant enzyme R44E, at 15°C, pH not specified in the publication | Escherichia coli | |
0.89 | - |
7,8-dihydrofolate | mutant enzyme R44Y, at 15°C, pH not specified in the publication | Escherichia coli | |
0.98 | - |
7,8-dihydrofolate | mutant enzyme R44L, at 15°C, pH not specified in the publication | Escherichia coli | |
1.15 | - |
7,8-dihydrofolate | mutant enzyme R44I, at 15°C, pH not specified in the publication | Escherichia coli | |
1.31 | - |
7,8-dihydrofolate | mutant enzyme R44V, at 15°C, pH not specified in the publication | Escherichia coli | |
1.39 | - |
7,8-dihydrofolate | mutant enzyme R44F, at 15°C, pH not specified in the publication | Escherichia coli | |
1.59 | - |
7,8-dihydrofolate | mutant enzyme R44H, at 15°C, pH not specified in the publication | Escherichia coli | |
1.78 | - |
7,8-dihydrofolate | mutant enzyme R44G, at 15°C, pH not specified in the publication | Escherichia coli | |
1.9 | - |
7,8-dihydrofolate | mutant enzyme R44Q, at 15°C, pH not specified in the publication | Escherichia coli | |
2 | - |
7,8-dihydrofolate | mutant enzyme R44T, at 15°C, pH not specified in the publication | Escherichia coli | |
2.41 | - |
7,8-dihydrofolate | mutant enzyme R44W, at 15°C, pH not specified in the publication | Escherichia coli | |
2.43 | - |
7,8-dihydrofolate | mutant enzyme R44C, at 15°C, pH not specified in the publication | Escherichia coli | |
3.05 | - |
7,8-dihydrofolate | mutant enzyme R44A, at 15°C, pH not specified in the publication | Escherichia coli | |
3.3 | - |
7,8-dihydrofolate | mutant enzyme R44N, at 15°C, pH not specified in the publication | Escherichia coli | |
3.8 | - |
7,8-dihydrofolate | mutant enzyme R44M, at 15°C, pH not specified in the publication | Escherichia coli | |
5.4 | - |
7,8-dihydrofolate | wild type enzyme, at 15°C, pH not specified in the publication | Escherichia coli | |
5.7 | - |
7,8-dihydrofolate | mutant enzyme R44K, at 15°C, pH not specified in the publication | Escherichia coli | |
5.8 | - |
7,8-dihydrofolate | mutant enzyme R44S, at 15°C, pH not specified in the publication | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
100 | - |
7,8-dihydrofolate | mutant enzyme R44P, at 15°C, pH not specified in the publication | Escherichia coli | |
230 | - |
7,8-dihydrofolate | mutant enzyme R44D, at 15°C, pH not specified in the publication | Escherichia coli | |
470 | - |
7,8-dihydrofolate | mutant enzyme R44E, at 15°C, pH not specified in the publication | Escherichia coli | |
500 | - |
7,8-dihydrofolate | mutant enzyme R44F, at 15°C, pH not specified in the publication | Escherichia coli | |
560 | - |
7,8-dihydrofolate | mutant enzyme R44I, at 15°C, pH not specified in the publication | Escherichia coli | |
660 | - |
7,8-dihydrofolate | mutant enzyme R44W, at 15°C, pH not specified in the publication | Escherichia coli | |
680 | - |
7,8-dihydrofolate | mutant enzyme R44L, at 15°C, pH not specified in the publication | Escherichia coli | |
800 | - |
7,8-dihydrofolate | mutant enzyme R44Q, at 15°C, pH not specified in the publication | Escherichia coli | |
800 | - |
7,8-dihydrofolate | mutant enzyme R44T, at 15°C, pH not specified in the publication | Escherichia coli | |
900 | - |
7,8-dihydrofolate | mutant enzyme R44Y, at 15°C, pH not specified in the publication | Escherichia coli | |
1200 | - |
7,8-dihydrofolate | mutant enzyme R44G, at 15°C, pH not specified in the publication | Escherichia coli | |
1300 | - |
7,8-dihydrofolate | mutant enzyme R44N, at 15°C, pH not specified in the publication | Escherichia coli | |
1600 | - |
7,8-dihydrofolate | mutant enzyme R44M, at 15°C, pH not specified in the publication | Escherichia coli | |
1700 | - |
7,8-dihydrofolate | mutant enzyme R44V, at 15°C, pH not specified in the publication | Escherichia coli | |
1900 | - |
7,8-dihydrofolate | mutant enzyme R44A, at 15°C, pH not specified in the publication | Escherichia coli | |
1900 | - |
7,8-dihydrofolate | mutant enzyme R44S, at 15°C, pH not specified in the publication | Escherichia coli | |
2200 | - |
7,8-dihydrofolate | mutant enzyme R44C, at 15°C, pH not specified in the publication | Escherichia coli | |
2300 | - |
7,8-dihydrofolate | mutant enzyme R44K, at 15°C, pH not specified in the publication | Escherichia coli | |
2500 | - |
7,8-dihydrofolate | mutant enzyme R44H, at 15°C, pH not specified in the publication | Escherichia coli | |
3400 | - |
7,8-dihydrofolate | wild type enzyme, at 15°C, pH not specified in the publication | Escherichia coli |