Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.3 extracted from

  • Labine, M.; DePledge, L.; Feirer, N.; Greenwich, J.; Fuqua, C.; Allen, K.
    Enzymatic and mutational analysis of the PruA pteridine reductase required for pterin-dependent control of biofilm formation in Agrobacterium tumefaciens (2020), J. Bacteriol., 202, e00098-20 .
    View publication on PubMed
Search for more literature for 1.5.1.3

Cloned(Commentary)

Cloned (Comment) Organism
gene ptr2, recombinant overexpression of N-terminally His6-tagged enzyme in Escherichia coli Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7,8-dihydrobiopterin + NADPH + H+ Trypanosoma cruzi
-
 5,6,7,8-tetrahydrobiopterin + NADP+
-
 ?
7,8-dihydrofolate + NADPH + H+ Trypanosoma cruzi best substrate 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q8I814
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, and gel filtration Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrobiopterin + NADPH + H+
-
 Trypanosoma cruzi 5,6,7,8-tetrahydrobiopterin + NADP+
-
 ?
7,8-dihydrobiopterin + NADPH + H+ high activity, see also EC 1.5.1.33 Trypanosoma cruzi 5,6,7,8-tetrahydrobiopterin + NADP+
-
 ?
7,8-dihydrofolate + NADPH + H+ best substrate Trypanosoma cruzi 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
additional information although presumably not its primary physiological functions, this SDR pteridine reductase also exhibits dihydrofolate reductase (DHFR) activity in vitro. Substrate specificity, overview. No activity with biopterin and folate Trypanosoma cruzi ?
-
-

Subunits

Subunits Comment Organism
homodimer
-
 Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
pteridine reductase
-
 Trypanosoma cruzi
PTR2
-
 Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Trypanosoma cruzi

General Information

General Information Comment Organism
evolution the enzyme belong to the short-chain dehydrogenase/reductase (SDR) family of enzymes. Despite the overall low sequence identity among members of the SDR family (about 15-30%), a central catalytic YX3K motif is highly conserved, as is an N-terminal glycine motif (TGX3GXG), involved in cofactor binding and recognition. The pteridine reductases in the SDR family have an arginine in place of the glycine at position 6 in this motif (TGX3RXG) Trypanosoma cruzi
metabolism pteridine reductase 1 (PTR1) is required for tetrahydrobiopterin (H4BPt) synthesis Trypanosoma cruzi
additional information enzyme structure modelling Trypanosoma cruzi
physiological function PTR1 produces tetrahydrobiopterin (H4BPt) from dihydrobiopterin (H2BPt) or from fully oxidized biopterin (BPt) scavenged from host cells Trypanosoma cruzi