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Literature summary for 1.5.1.37 extracted from

  • Fang, L.; Qin, H.; Shi, T.; Wu, X.; Li, Q.; Hua, R.
    Ortho and para oxydehalogenation of dihalophenols catalyzed by the monooxygenase TcpA and NAD(P)H FAD reductase Fre (2020), J. Hazard. Mater., 388, 121787 .
    View publication on PubMed
Search for more literature for 1.5.1.37

Application

Application Comment Organism
environmental protection strain X1 Fre can effectively dehalogenate dihalophenols, which can be useful for the treatment of dihalophenols in wastewaters and remediation of DCP-contaminated environments Cupriavidus nantongensis

Cloned(Commentary)

Cloned (Comment) Organism
gene fre, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Cupriavidus nantongensis

Organism

Organism UniProt Comment Textmining
Cupriavidus nantongensis
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Cupriavidus nantongensis X1
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme Fre from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration Cupriavidus nantongensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Fre can effectively dehalogenate dihalophenols. Degradation of dichlorophenol (DCP) structural analogues by recombinant 2,4,6-trichlorophenol monooxygenase (TcpA) with NAD(P)H:FAD reductase (Fre), substrate specificities with 2,3-dichlorophenol (2,3-DCP), 2,4-dichlorophenol (2,4-DCP), 2,5-dichlorophenol (2,5-DCP), 2,6-dichlorophenol (2,6-DCP), 3,4-dichlorophenol (3,4 DCP), and 3,5-dichlorophenol (3,5-DCP), overview Cupriavidus nantongensis ?
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additional information Fre can effectively dehalogenate dihalophenols. Degradation of dichlorophenol (DCP) structural analogues by recombinant 2,4,6-trichlorophenol monooxygenase (TcpA) with NAD(P)H:FAD reductase (Fre), substrate specificities with 2,3-dichlorophenol (2,3-DCP), 2,4-dichlorophenol (2,4-DCP), 2,5-dichlorophenol (2,5-DCP), 2,6-dichlorophenol (2,6-DCP), 3,4-dichlorophenol (3,4 DCP), and 3,5-dichlorophenol (3,5-DCP), overview Cupriavidus nantongensis X1 ?
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additional information Fre can effectively dehalogenate dihalophenols. Degradation of dichlorophenol (DCP) structural analogues by recombinant 2,4,6-trichlorophenol monooxygenase (TcpA) with NAD(P)H:FAD reductase (Fre), substrate specificities with 2,3-dichlorophenol (2,3-DCP), 2,4-dichlorophenol (2,4-DCP), 2,5-dichlorophenol (2,5-DCP), 2,6-dichlorophenol (2,6-DCP), 3,4-dichlorophenol (3,4 DCP), and 3,5-dichlorophenol (3,5-DCP), overview Cupriavidus nantongensis X1T ?
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Synonyms

Synonyms Comment Organism
fre
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 Cupriavidus nantongensis
NAD(P)H:FAD reductase
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 Cupriavidus nantongensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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 assay at Cupriavidus nantongensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
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 assay at Cupriavidus nantongensis

General Information

General Information Comment Organism
additional information the structure and active site of Fre is constructed and analyzed via homologous modeling and molecular docking Cupriavidus nantongensis