General Stability | Organism |
---|---|
freezing inactivates | Moorella thermoacetica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
NADP+ | - |
Moorella thermoacetica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, SDS-PAGE | Moorella thermoacetica |
55000 | - |
methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase combined, equilibrium ultracentrifugation | Moorella thermoacetica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADP+ | Moorella thermoacetica | - |
5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase combined | Moorella thermoacetica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
310 | - |
assays at 37°C | Moorella thermoacetica |
720 | - |
at 60°C growth temperature | Moorella thermoacetica |
Storage Stability | Organism |
---|---|
4°C, stable for at least a month | Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADP+ | - |
Moorella thermoacetica | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, SDS-PAGE | Moorella thermoacetica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
64 | - |
above | Moorella thermoacetica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Moorella thermoacetica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Moorella thermoacetica |