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Literature summary for 1.5.1.6 extracted from

  • Kim, S.; Park, G.H.; Joo, W.A.; Paik, W.K.; Cook, R.J.; Williams, K.R.
    Identification of protein-arginine N-methyltransferase as 10-formyltetrahydrofolate dehydrogenase (1998), J. Biol. Chem., 273, 27374-27382.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol optimal dehydrogenase activity requires 15 mM or higher concentrations Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression of FDH in insect cells using the pVL 1393 baculovirus expression vector Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information recombinant FDH is not inhibited by sodium azide Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106000
-
4 * 106000, SDS-PAGE Rattus norvegicus
450000
-
nondenaturing PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O Rattus norvegicus regulatory mechanism to control the in vivo folate pool size tetrahydrofolate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant liver FDH Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.094
-
recombinant FDH, 10-formyltetrahydrofolate as substrate Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyl-5,8-dideazafolate + NADP+ + H2O
-
Rattus norvegicus 5,8-dideazafolate + CO2 + NADPH + H+
-
?
10-formyltetrahydrofolate + NADP+ + H2O
-
Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
?
10-formyltetrahydrofolate + NADP+ + H2O regulatory mechanism to control the in vivo folate pool size Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
?
additional information enzyme exhibit additional to 10-formyltetrahydrofolate dehydrogenase/hydrolase activities NADP+-dependent aldehyde dehydrogenase activity with propanal as preferred substrate Rattus norvegicus ?
-
?
additional information FDH has also methyltransferase activity Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 106000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
More identical with protein-arginine methyltransferase from liver cytosol, EC 2.1.1.23 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
folate 10-formyl-5,8-dideazafolate and 5,8-dideazafolate Rattus norvegicus
NADP+ NADP+-dependent Rattus norvegicus
tetrahydrofolate
-
Rattus norvegicus