Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Corynebacterium sp. P-1 |
Protein Variants | Comment | Organism |
---|---|---|
C141S | the mutant exhibits specific activity values reduced to those of the wild type recombinant enzyme or the natural enzyme | Corynebacterium sp. P-1 |
C195S | the mutant exhibits specific activity values similar to those of the wild type recombinant enzyme or the natural enzyme | Corynebacterium sp. P-1 |
C351S | the mutant exhibits specific activity values similar to those of the wild type recombinant enzyme or the natural enzyme | Corynebacterium sp. P-1 |
G139A | the mutant of the alpha subunit is expressed only at low temperature (15°C), but the purified enzyme exhibits properties indistinguishable from the wild type enzyme | Corynebacterium sp. P-1 |
G30A | the mutant of the beta subunit exhibits subunit expression levels similar to those of the wild type enzyme, but the mutation blocks subunit assembly and covalent attachment of FMN | Corynebacterium sp. P-1 |
H173N | catalytically inactive mutant of the beta subunit | Corynebacterium sp. P-1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + 5,6,7,8-tetrahydrofolate + O2 | Corynebacterium sp. P-1 | - |
glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. P-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phenyl-Sepharose column chromatography and Talon affinity resin column chromatography | Corynebacterium sp. P-1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylthioacetate + 5,6,7,8-tetrahydrofolate + O2 | - |
Corynebacterium sp. P-1 | ? | - |
? | |
sarcosine + 5,6,7,8-tetrahydrofolate + O2 | - |
Corynebacterium sp. P-1 | glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | - |
Corynebacterium sp. P-1 |
Synonyms | Comment | Organism |
---|---|---|
heterotetrameric sarcosine oxidase | - |
Corynebacterium sp. P-1 |
TSOX | - |
Corynebacterium sp. P-1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Corynebacterium sp. P-1 | |
FMN | - |
Corynebacterium sp. P-1 | |
NAD+ | - |
Corynebacterium sp. P-1 |