BRENDA - Enzyme Database
show all sequences of 1.5.99.5

Genetics of the glutamate-mediated methylamine utilization pathway in the facultative methylotrophic beta-proteobacterium Methyloversatilis universalis FAM5

Latypova, E.; Yang, S.; Wang, Y.S.; Wang, T.; Chavkin, T.A.; Hackett, M.; Schaefer, H.; Kalyuzhnaya, M.G.; Mol. Microbiol. 75, 426-439 (2010) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
genes mgdA, mgdB, and mgdC, encoding subunits A, B, and large subunit c, the enzyme is encoded in a gene cluster essential for operation of the N-methylglutamate pathway, genetic structure
Methyloversatilis universalis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N-methyl-L-glutamate + acceptor + H2O
Methyloversatilis universalis
-
L-glutamate + formaldehyde + reduced acceptor
-
-
?
N-methyl-L-glutamate + acceptor + H2O
Methyloversatilis universalis FAM5
-
L-glutamate + formaldehyde + reduced acceptor
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Methyloversatilis universalis
-
genes mgdA, mgdB, and mgdC, encoding subunits A, B, and large subunit c
-
Methyloversatilis universalis FAM5
-
genes mgdA, mgdB, and mgdC, encoding subunits A, B, and large subunit c
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
N-methyl-L-glutamate + acceptor + H2O
-
700286
Methyloversatilis universalis
L-glutamate + formaldehyde + reduced acceptor
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
-
700286
Methyloversatilis universalis FAM5
L-glutamate + formaldehyde + reduced acceptor
-
-
-
?
N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
700286
Methyloversatilis universalis
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
700286
Methyloversatilis universalis FAM5
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
subunits A, B, and large subunit c
Methyloversatilis universalis
Synonyms
Synonyms
Commentary
Organism
N-methyl-L-glutamate:oxidoreductase
-
Methyloversatilis universalis
N-methylglutamate dehydrogenase
-
Methyloversatilis universalis
NMGDH
-
Methyloversatilis universalis
Cloned(Commentary) (protein specific)
Commentary
Organism
genes mgdA, mgdB, and mgdC, encoding subunits A, B, and large subunit c, the enzyme is encoded in a gene cluster essential for operation of the N-methylglutamate pathway, genetic structure
Methyloversatilis universalis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N-methyl-L-glutamate + acceptor + H2O
Methyloversatilis universalis
-
L-glutamate + formaldehyde + reduced acceptor
-
-
?
N-methyl-L-glutamate + acceptor + H2O
Methyloversatilis universalis FAM5
-
L-glutamate + formaldehyde + reduced acceptor
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
N-methyl-L-glutamate + acceptor + H2O
-
700286
Methyloversatilis universalis
L-glutamate + formaldehyde + reduced acceptor
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
-
700286
Methyloversatilis universalis FAM5
L-glutamate + formaldehyde + reduced acceptor
-
-
-
?
N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
700286
Methyloversatilis universalis
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
700286
Methyloversatilis universalis FAM5
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
subunits A, B, and large subunit c
Methyloversatilis universalis
General Information
General Information
Commentary
Organism
metabolism
SoxBDAG, encoding sarcosine dehydrogenase,may be involved in oxidation of N-methyl-L-glutamate, overview
Methyloversatilis universalis
additional information
the enzyme is encoded in a gene cluster essential for operation of the N-methylglutamate pathway in the methylotrophic beta-proteobacterium Methyloversatilis universalis FAM5. The enzyme contains an aminomethyltransferase folate-binding domain near the C-terminus of its large subunit
Methyloversatilis universalis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
SoxBDAG, encoding sarcosine dehydrogenase,may be involved in oxidation of N-methyl-L-glutamate, overview
Methyloversatilis universalis
additional information
the enzyme is encoded in a gene cluster essential for operation of the N-methylglutamate pathway in the methylotrophic beta-proteobacterium Methyloversatilis universalis FAM5. The enzyme contains an aminomethyltransferase folate-binding domain near the C-terminus of its large subunit
Methyloversatilis universalis
Other publictions for EC 1.5.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
700286
Latypova
Genetics of the glutamate-medi ...
Methyloversatilis universalis, Methyloversatilis universalis FAM5
Mol. Microbiol.
75
426-439
2010
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723999
Chen
gamma-Glutamylmethylamide is a ...
Methylocella silvestris, Methylocella silvestris BL2
Appl. Environ. Microbiol.
76
4530-4537
2010
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1
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1
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1
1
1
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437750
Biville
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Physiological properties of a ...
Methylobacterium organophilum
FEMS Microbiol. Lett.
52
53-58
1988
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437749
Janvier
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Methylophaga marina gen nov., ...
Methylophaga marina, Methylophaga thalassica
Int. J. Syst. Bacteriol.
35
131-139
1985
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437748
Boulton
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N-Methylglutamate dehydrogenas ...
bacterium AT2, Hyphomicrobium vulgare, Pseudomonas sp., Aminobacter aminovorans, Pseudomonas methylica, Aminobacter aminovorans AM1, Pseudomonas sp. MA / M.A. / ATCC 23819, Hyphomicrobium vulgare ZV, Hyphomicrobium vulgare 3
J. Gen. Microbiol.
117
293-304
1980
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18
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2
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6
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39
2
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1
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1
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437747
Bamforth
The kinetic mechanism of N-met ...
Aminobacter aminovorans
Biochem. Soc. Trans.
6
193-195
1978
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1
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437745
Bamforth
Solubilization, partial purifi ...
Aminobacter aminovorans, Aminobacter aminovorans AM1, Aminobacter aminovorans N.C.I.B. 9039
Biochem. J.
161
357-370
1977
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6
7
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13
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2
19
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2
19
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437746
Bamforth
The molecular size of N-methyl ...
Aminobacter aminovorans
Biochem. J.
167
509-512
1977
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437744
Bamforth
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Solubilization and partial pur ...
Pseudomonas sp., Aminobacter aminovorans, Aminobacter aminovorans AM1, Pseudomonas sp. MA / M.A. / ATCC 23819
Biochem. Soc. Trans.
3
1066-1069
1975
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3
7
2
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3
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13
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23
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23
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437743
Hersh
N-methylglutamate dehydrogenas ...
Pseudomonas sp., Pseudomonas sp. MA / M.A. / ATCC 23819
Arch. Biochem. Biophys.
150
219-226
1972
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12
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19
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437742
Hersh
An N-methyl glutamate dehydrog ...
Pseudomonas sp., Pseudomonas sp. MA / M.A. / ATCC 23819
Arch. Biochem. Biophys.
145
115-120
1971
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