Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2'-AMP | activation of reduction of NADP+ or thio-NAD+ by NADH, no effect on reduction of NAD+ or thio-NAD+ by NADPH | Azotobacter vinelandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | competitive to thio-NAD+, uncompetitive with respect to NADPH | Azotobacter vinelandii | |
NADP+ | uncompetitive to thio-NAD+ | Azotobacter vinelandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
thio-NAD+ | cosubstrate NADH | Azotobacter vinelandii | |
0.077 | - |
NADH | + thio-NAD+ | Azotobacter vinelandii | |
0.25 | - |
thio-NAD+ | cosubstrate NADPH | Azotobacter vinelandii | |
0.38 | - |
NAD+ | cosubstrate NADPH | Azotobacter vinelandii | |
0.4 | - |
deamino-NAD+ | cosubstrate NADPH | Azotobacter vinelandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
251 | - |
- |
Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADP+ + NADH | - |
Azotobacter vinelandii | NADPH + NAD+ | - |
? | |
NADPH + deamino-NAD+ | - |
Azotobacter vinelandii | NADP+ + deamino-NADH | - |
? | |
NADPH + thio-NADP+ + H+[side 1] | - |
Azotobacter vinelandii | NADP+ + thio-NADPH + H+[side 2] | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Azotobacter vinelandii |