Cloned (Comment) | Organism |
---|---|
gene Nnt, overexpression of C-terminally V5-tagged full-length cDNA in the macrophage-like cell line RAW264.7, real-time PCR expression analysis | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme overexpression reduces the production of inflammatory cytokines and the activation of mitogen-activated protein kinase signaling pathways in response to lipopolysaccharides. After 8 h, RAW264.7/NNT cells secrete significantly lower amounts of interleukin 6, TNF-alpha, and IL-1beta when compared to cells expressing empty vector. NNT overexpression impairs intracellular bacteria clearance. Wild-type C57BL/6J and C57BL/6J-NNT mice differed in inflammatory response within lung tissue | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Mus musculus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
gene Nnt | - |
Mus musculus C3H/HeJ | - |
gene Nnt | - |
Mus musculus C57/BL6J | - |
gene Nnt | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow | high expression level | Mus musculus | - |
brain | - |
Mus musculus | - |
heart | - |
Mus musculus | - |
kidney | - |
Mus musculus | - |
liver | high expression level | Mus musculus | - |
lung | high expression level | Mus musculus | - |
macrophage | bone marrow-derived, Nnt mRNA and protein are expressed in resting macrophages and down-regulated in response to inflammatory stimuli such as lipopolysaccharide. Nnt mRNA and protein expression are further repressed in fully activated macrophages | Mus musculus | - |
macrophage | bone marrow-derived, Nnt mRNA and protein are expressed in resting macrophages and down-regulated in response to inflammatory stimuli such as lipopolysaccharide. Nnt mRNA and protein expression are repressed in fully activated macrophages | Mus musculus | - |
additional information | quantitative PCR protein expression analysis | Mus musculus | - |
pancreas | - |
Mus musculus | - |
RAW-264.7 cell | - |
Mus musculus | - |
skeletal muscle | - |
Mus musculus | - |
spleen | high expression level | Mus musculus | - |
Synonyms | Comment | Organism |
---|---|---|
nicotinamide nucleotide transhydrogenase | - |
Mus musculus |
NNT | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | C57BL/6J mice, which have a deletion in the Nnt gene, exhibit greater resistance to acute pulmonary infection with Streptococcus pneumoniae. Macrophages from these mice generate more reactive oxygen species and establish a stronger inflammatory response to this pathogen | Mus musculus |
physiological function | nicotinamide nucleotide transhydrogenase, NNT, is a nuclear-encoded protein that functions as a redox-driven proton pump catalyzing the reversible reduction of NADP+ by NADH and conversion of NADH to NAD+. The enzyme acts as a modulator of macrophage inflammatory responses. The enzyme NNT is an efficient generator of NADPH required by glutathione reductase to reduce glutathione, a component necessary to decrease the buildup of reactive oxygen species | Mus musculus |