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Literature summary for 1.6.3.1 extracted from

  • Magnani, F.; Nenci, S.; Millana Fananas, E.; Ceccon, M.; Romero, E.; Fraaije, M.W.; Mattevi, A.
    Crystal structures and atomic model of NADPH oxidase (2017), Proc. Natl. Acad. Sci. USA, 114, 6764-6769 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-RP plus cells Cylindrospermum stagnale

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 160 mM Ca-acetate, 80 mM Na-Cacodylate pH 6.5, 12.16% (w/v) PEG 8000, 20% (v/v) glycerol Cylindrospermum stagnale

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADPH + H+ + O2 Cylindrospermum stagnale
-
 NADP+ + H2O2
-
 ?
NADPH + H+ + O2 Cylindrospermum stagnale PCC 7417
-
 NADP+ + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Cylindrospermum stagnale K9WT99
-
-
Cylindrospermum stagnale PCC 7417 K9WT99
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep column chromatography, MonoQ column chromatography, and Superdex 75 gel filtration Cylindrospermum stagnale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + O2
-
 Cylindrospermum stagnale NADP+ + H2O2
-
 ?
NADPH + H+ + O2
-
 Cylindrospermum stagnale PCC 7417 NADP+ + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
NADPH oxidase
-
 Cylindrospermum stagnale
NOX5
-
 Cylindrospermum stagnale

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Cylindrospermum stagnale
heme
-
 Cylindrospermum stagnale
NADPH
-
 Cylindrospermum stagnale