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Literature summary for 1.7.1.3 extracted from

  • Nason, A.; Evans, H.J.
    Triphosphopyridine nucleotide-nitrate reductase in Neurospora (1953), J. Biol. Chem., 202, 655-673.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline
-
Neurospora crassa
azide
-
Neurospora crassa
Cu2+ inhibitory Neurospora crassa
cyanide
-
Neurospora crassa
o-phenanthroline
-
Neurospora crassa
p-chloromercuribenzoate reversed by cysteine or glutathione Neurospora crassa
potassium ethyl xanthate
-
Neurospora crassa
Thiourea
-
Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + NADPH Neurospora crassa
-
nitrite + NADP+ + H2O
-
?
nitrate + NADPH Neurospora crassa 5297a
-
nitrite + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
5297a
-
Neurospora crassa 5297a
-
5297a
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Neurospora crassa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
-
one unit is defined as that amount of enzyme which results in the formation of 0.000001 mM of nitrite Neurospora crassa

Storage Stability

Storage Stability Organism
-15┬░C, fraction II, most stable, optimal pH: 7.0, 10-20% loss of activity after a month Neurospora crassa
-15┬░C, fraction IV, one week, loses about half of its activity Neurospora crassa
-15┬░C, fraction V, quite unstable, overnight, loses at least half of its activity Neurospora crassa
4┬░C or -15┬░C, fraction III, overnight, 50% loss of activity Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADPH
-
Neurospora crassa nitrite + NADP+ + H2O
-
?
nitrate + NADPH 20-fold higher activity with NADPH than with NADH Neurospora crassa nitrite + NADP+ + H2O
-
ir
nitrate + NADPH
-
Neurospora crassa 5297a nitrite + NADP+ + H2O
-
?
nitrate + NADPH 20-fold higher activity with NADPH than with NADH Neurospora crassa 5297a nitrite + NADP+ + H2O
-
ir

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
23 28 assay at Neurospora crassa

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
40
-
fraction V, pH 7.0, 50% loss of activity within 5 min Neurospora crassa
50
-
fraction V, pH 7.0, loses all of its activity in 5 min Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Neurospora crassa

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein, prosthetic group Neurospora crassa
FAD stimulated by flavin Neurospora crassa
FMN to a lesser extent than FAD Neurospora crassa
additional information cytochrome a or b involved Neurospora crassa