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Literature summary for 1.7.1.3 extracted from

  • Antoine, A.D.
    Purification and properties of the nitrate reductase isolated from Neurospora crassa mutant nit-3. Kinetics, molecular weight determination, and cytochrome involvement (1974), Biochemistry, 13, 2289-2294.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information nit-3 mutant (FGSC 262): reduced FAD-nitrate reductase and reduced methylviologen-nitrate reductase activities Neurospora crassa

Inhibitors

Inhibitors Comment Organism Structure
p-hydroxymercuribenzoate at 0.1 mM complete inhibition Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
235000
-
sucrose density gradient centrifugation, gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + NADPH Neurospora crassa
-
nitrite + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
wild-type STA4, FGSC 358 and nit-1 mutant, FGSC 262
-

Purification (Commentary)

Purification (Comment) Organism
partially purified Neurospora crassa

Reaction

Reaction Comment Organism Reaction ID
nitrite + NADP+ + H2O = nitrate + NADPH + H+ sulfhydryl groups may participate in the binding of the protein subunits Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
8.55
-
-
Neurospora crassa

Storage Stability

Storage Stability Organism
-20┬░C, stable for 6 months or longer, 5% loss of activity Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADPH
-
Neurospora crassa nitrite + NADP+ + H2O
-
?
nitrate + NADPH associated FAD-nitrate reductase and methylviologen-nitrate activity Neurospora crassa nitrite + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
More at least 2 subunits suggested Neurospora crassa

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
60
-
labile, loss of acitvity after 2 min Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
optima of associated activities Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
cytochrome b557
-
Neurospora crassa
FAD
-
Neurospora crassa