Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.1.6 extracted from

  • Rathod, J.; Dhebar, S.; Archana, G.
    Efficient approach to enhance whole cell azo dye decolorization by heterologous overexpression of Enterococcus sp. L2 azoreductase (azoA) and Mycobacterium vaccae formate dehydrogenase (fdh) in different bacterial systems (2017), Int. Biodeter. Biodegrad., 124, 91-100 .
No PubMed abstract available
Search for more literature for 1.7.1.6

Cloned(Commentary)

Cloned (Comment) Organism
gene azoA, subcloning in Escherichia coli strain DH5alpha, and constitutive recombinant expression in Pseudomonas fluorescens strain PfO-1 using vector pBBRMCS2 leading to 2fold increased azoreductase activity, coexpression of Mycobacterium vaccae strain N10 NAD+-dependent formate dehydrogenase gene. FDH results in a 3.5-4fold increased dye decolorization, thus developing an in vivo NADH regeneration system using formate as an electron donor. Overexpression of both genes azoA and fdh via transcription fusion under plac and/or pT7 promoters in Escherichia coli strain BL21 (DE3) and Pseudomonas fluorescens strain PfO-1 further increases the RV5R decolorization in formate constituted buffered conditions compared to only azoA or fdh overexpression Enterococcus sp.

Organism

Organism UniProt Comment Textmining
Enterococcus sp.
-
-
-
Enterococcus sp. L2
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acid red 119 + NADH + H+
-
 Enterococcus sp. ? + NAD+
-
 ?
acid red 119 + NADH + H+
-
 Enterococcus sp. L2 ? + NAD+
-
 ?
mordant blue 13 + NADH + H+
-
 Enterococcus sp. ? + NAD+
-
 ?
mordant blue 13 + NADH + H+
-
 Enterococcus sp. L2 ? + NAD+
-
 ?
additional information the enzyme from strain L2 decolorizes a wide spectrum of azo dyes with a constantly high activity, overview Enterococcus sp. ?
-
 ?
additional information the enzyme from strain L2 decolorizes a wide spectrum of azo dyes with a constantly high activity, overview Enterococcus sp. L2 ?
-
 ?
reactive black B5 + NADH + H+
-
 Enterococcus sp. ? + NAD+
-
 ?
reactive black B5 + NADH + H+
-
 Enterococcus sp. L2 ? + NAD+
-
 ?
reactive blue 222 + NADH + H+
-
 Enterococcus sp. ? + NAD+
-
 ?
reactive blue 222 + NADH + H+
-
 Enterococcus sp. L2 ? + NAD+
-
 ?
reactive blue 3R + NADH + H+
-
 Enterococcus sp. ? + NAD+
-
 ?
reactive orange 122 + NADH + H+
-
 Enterococcus sp. ? + NAD+
-
 ?
reactive red 195 + NADH + H+
-
 Enterococcus sp. ? + NAD+
-
 ?
reactive violet 5R + NADH + H+
-
 Enterococcus sp. ? + NAD+
-
 ?
reactive yellow 186 + NADH + H+ low activity Enterococcus sp. ? + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
AzoA
-
 Enterococcus sp.
azoreductase
-
 Enterococcus sp.
FMN-dependent NADH-azoreductase UniProt Enterococcus sp.
NADH driven FMN dependent azoreductase
-
 Enterococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Enterococcus sp.

Cofactor

Cofactor Comment Organism Structure
FMN dependent on Enterococcus sp.
NADH
-
 Enterococcus sp.

General Information

General Information Comment Organism
physiological function the rate limiting first step of the biodegradation pathway of recalcitrant azo dyes is their decolorization, which involves the reductive cleavage of the azo bond catalysed by the enzyme azoreductase. The enzyme from strain L2 decolorizes a wide spectrum of azo dyes Enterococcus sp.