Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | slight | Shewanella sp. | |
2,2-dipyridyl | slight | Shewanella sp. | |
5,5'-dithiobis(2-nitrobenzoate) | - |
Shewanella sp. | |
iodoacetate | partial | Shewanella sp. | |
KCN | partial | Shewanella sp. | |
Na2MoO4 | - |
Shewanella sp. | |
Na2WO4 | - |
Shewanella sp. | |
p-chloromercuribenzoate | - |
Shewanella sp. | |
Sodium chlorate | partial | Shewanella sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Trimethylamine N-oxide | - |
Shewanella sp. | |
2.41 | - |
N,N-dimethyldodecylamine N-oxide | - |
Shewanella sp. | |
6.95 | - |
gamma-picoline N-oxide | - |
Shewanella sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Shewanella sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | enzyme contains 1.32 mol molybdenum per mol of enzyme | Shewanella sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 MW forms, MW: 47000, SDS-PAGE in absence of reducing agents and MW: 84000, gel filtration, SDS-PAGE under reducing conditions | Shewanella sp. |
84000 | - |
2 MW forms, MW: 47000, SDS-PAGE in absence of reducing conditions and MW: 84000, gel filtration, SDS-PAGE under reducing conditions | Shewanella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella sp. | - |
marine bacterium, 2 enzyme forms | - |
Purification (Comment) | Organism |
---|---|
of 2 enzyme forms, using ammonium sulfate treatment, ion exchange buffer treatment, column chromatography on DEAE-Sepharose CL6B and hydroxyapatite and gel filtration on Sephacryl S-300 | Shewanella sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
211.5 | - |
- |
Shewanella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine N-oxide + electron donor | 40% relative activity to trimethylamine N-oxide with the periplasmic enzyme and 35% relative activity to trimethylamine N-oxide with the purified enzyme | Shewanella sp. | adenosine + H2O + oxidized electron donor | - |
? | |
gamma-picoline N-oxide + electron donor | 11% relative activity to trimethylamine N-oxide with the periplasmic enzyme and 12% relative activity to trimethylamine N-oxide with the purified enzyme | Shewanella sp. | gamma-picoline + H2O + oxidized electron donor | - |
? | |
hydroxylamine N-oxide + electron donor | 19% relative activity to trimethylamine N-oxide with the periplasmic enzyme and 3% relative activity to trimethylamine N-oxide with the purified enzyme | Shewanella sp. | hydroxylamine + H2O + oxidized electron donor | - |
? | |
additional information | no activity with FADH2, NADH, NADPH and the reduced forms of methylene blue and N-methylphenazonium methosulphate | Shewanella sp. | ? | - |
? | |
N,N-dimethyldodecylamine N-oxide + electron donor | 63% relative activity to trimethylamine N-oxide with the periplasmic enzyme and 41% relative activity to trimethylamine N-oxide with the purified enzyme | Shewanella sp. | N,N-dimethyldodecylamine + H2O + oxidized electron donor | - |
? | |
trimethylamine N-oxide + electron donor | methyl viologen as electron donor | Shewanella sp. | trimethylamine + oxidized electron donor + H2O | - |
? | |
trimethylamine N-oxide + electron donor | reduced methylene blue, reduced N-methylphenazonium methosulfate as electron donors | Shewanella sp. | trimethylamine + oxidized electron donor + H2O | - |
? | |
trimethylamine N-oxide + electron donor | benzyl viologen as electron donor | Shewanella sp. | trimethylamine + oxidized electron donor + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 84000, SDS-PAGE under reducing conditions | Shewanella sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | stimulation | Shewanella sp. | |
FMN | stimulation | Shewanella sp. |