BRENDA - Enzyme Database
show all sequences of 1.7.2.4

Purification, Characterization, and Preliminary Crystallographic Study of Copper-Containing Nitrous Oxide Reductase from Pseudomonas nautica 617

Prudencio, M.; Pereira, A.S.; Tavares, P.; Besson, S.; Cabrito, I.; Brown, K.; Samyn, B.; Devreese, B.; Van Beeumen, J.; Rusnak, F.; Fauque, G.; Moura, J.J.G.; Tegoni, M.; Cambillau, C.; Moura, I.; Biochemistry 39, 3899-3907 (2000)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
2.4 A resolution, preliminary study
Marinobacter hydrocarbonoclasticus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu
six copper atoms per monomer arranged in two centers named CuA and CuZ, 10.7 atoms per dimer
Marinobacter hydrocarbonoclasticus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
2 * 65000, SDS-PAGE
Marinobacter hydrocarbonoclasticus
65373
-
2 * 65373, ESI mass spectrometry
Marinobacter hydrocarbonoclasticus
120000
-
gel filtration
Marinobacter hydrocarbonoclasticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
nitrous oxide + reduced acceptor
Marinobacter hydrocarbonoclasticus
-
nitrogen + H2O + acceptor
-
-
?
nitrous oxide + reduced acceptor
Marinobacter hydrocarbonoclasticus 617
-
nitrogen + H2O + acceptor
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Marinobacter hydrocarbonoclasticus
-
-
-
Marinobacter hydrocarbonoclasticus 617
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
two forms a purple and a blue form
Marinobacter hydrocarbonoclasticus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
23
-
blue-coloured form
Marinobacter hydrocarbonoclasticus
55
-
purple coloured form
Marinobacter hydrocarbonoclasticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
nitrous oxide + reduced acceptor
-
394519
Marinobacter hydrocarbonoclasticus
nitrogen + H2O + acceptor
-
-
-
?
nitrous oxide + reduced acceptor
-
394519
Marinobacter hydrocarbonoclasticus 617
nitrogen + H2O + acceptor
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 65000, SDS-PAGE; 2 * 65373, ESI mass spectrometry
Marinobacter hydrocarbonoclasticus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
2.4 A resolution, preliminary study
Marinobacter hydrocarbonoclasticus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu
six copper atoms per monomer arranged in two centers named CuA and CuZ, 10.7 atoms per dimer
Marinobacter hydrocarbonoclasticus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
2 * 65000, SDS-PAGE
Marinobacter hydrocarbonoclasticus
65373
-
2 * 65373, ESI mass spectrometry
Marinobacter hydrocarbonoclasticus
120000
-
gel filtration
Marinobacter hydrocarbonoclasticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
nitrous oxide + reduced acceptor
Marinobacter hydrocarbonoclasticus
-
nitrogen + H2O + acceptor
-
-
?
nitrous oxide + reduced acceptor
Marinobacter hydrocarbonoclasticus 617
-
nitrogen + H2O + acceptor
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
two forms a purple and a blue form
Marinobacter hydrocarbonoclasticus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
23
-
blue-coloured form
Marinobacter hydrocarbonoclasticus
55
-
purple coloured form
Marinobacter hydrocarbonoclasticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
nitrous oxide + reduced acceptor
-
394519
Marinobacter hydrocarbonoclasticus
nitrogen + H2O + acceptor
-
-
-
?
nitrous oxide + reduced acceptor
-
394519
Marinobacter hydrocarbonoclasticus 617
nitrogen + H2O + acceptor
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 65000, SDS-PAGE; 2 * 65373, ESI mass spectrometry
Marinobacter hydrocarbonoclasticus
Other publictions for EC 1.7.2.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742390
Sanchez
Regulation of nitrous oxide r ...
Bradyrhizobium diazoefficiens
Environ. Microbiol. Rep.
9
389-396
2017
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1
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1
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1
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-
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-
-
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3
2
2
3
-
-
742753
Johnston
Spectroscopic definition of t ...
Marinobacter hydrocarbonoclasticus, Marinobacter hydrocarbonoclasticus 617
J. Am. Chem. Soc.
139
4462-4476
2017
-
-
-
-
-
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1
-
1
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3
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1
1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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4
-
1
-
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1
-
-
-
-
-
-
-
-
-
741924
Schneider
Role of calcium in secondary ...
Shewanella denitrificans, Shewanella denitrificans OS217
Biochemistry
55
1433-1440
2016
-
-
1
1
-
-
-
-
1
2
1
2
-
4
-
-
1
-
-
-
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-
4
2
6
-
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1
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1
1
1
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1
2
1
2
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1
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-
4
2
-
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-
-
-
-
-
-
-
2
2
-
-
-
742384
Qu
Transcriptional and metabolic ...
Paracoccus denitrificans
Environ. Microbiol.
18
2951-2963
2016
-
-
1
-
-
-
1
-
-
1
-
1
-
3
1
-
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-
1
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2
-
3
-
-
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1
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1
1
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1
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1
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1
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1
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1
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-
2
-
-
-
-
-
-
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-
1
1
1
1
-
-
741746
Zhang
Response of denitrifying gene ...
uncultured bacterium
Appl. Microbiol. Biotechnol.
99
4059-4070
2015
-
-
1
-
-
-
-
-
-
-
-
-
-
1
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2
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1
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-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
742272
Johnston
Protonation state of the Cu4S ...
Paracoccus pantotrophus, Pseudomonas stutzeri
Chem. Sci.
6
5670-5679
2015
-
-
-
-
-
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-
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2
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3
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-
-
741677
Wyman
Denitrifying alphaproteobacte ...
alpha proteobacterium 4N, Trichodesmium erythraeum, Trichodesmium erythraeum IMS01, uncultured Alphaproteobacteria bacterium
Appl. Environ. Microbiol.
79
2670-2681
2013
-
-
3
-
-
-
-
-
-
-
-
4
-
8
-
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-
-
-
-
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4
-
9
-
-
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3
-
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3
3
-
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4
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-
4
-
-
-
-
-
-
-
-
-
-
6
6
-
-
-
725551
Ertem
N-O bond cleavage mechanism(s) ...
Paracoccus denitrificans
J. Biol. Inorg. Chem.
17
687-698
2012
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1
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-
725648
Fujita
Direct electron transfer from ...
Achromobacter cycloclastes
J. Inorg. Biochem.
115
163-173
2012
-
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3
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1
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4
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1
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3
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1
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2
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-
2
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-
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-
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-
726100
DellAcqua
Biochemical characterization o ...
Marinobacter hydrocarbonoclasticus
Philos. Trans. R. Soc. Lond. B Biol. Sci.
367
1204-1212
2012
-
-
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-
-
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2
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1
1
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4
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2
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1
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1
1
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4
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2
-
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-
-
-
-
-
-
726523
Wan
Expression of the nos operon p ...
Pseudomonas stutzeri, Pseudomonas stutzeri ATCC 14405
Transgenic Res.
21
593-603
2012
-
1
1
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5
-
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1
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2
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1
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1
1
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1
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2
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724958
Fernandes
The multicopper oxidase from t ...
Pyrobaculum aerophilum
FEBS J.
277
3176-3189
2010
-
-
1
1
4
-
-
2
-
1
3
-
-
5
-
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2
1
2
1
-
1
1
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1
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1
4
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2
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1
3
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2
1
1
-
1
1
-
-
-
-
-
-
-
-
2
2
700984
Savelieff
Experimental evidence for a li ...
Paracoccus denitrificans
Proc. Natl. Acad. Sci. USA
105
7919-7924
2008
-
-
1
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1
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3
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1
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1
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1
1
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1
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1
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698268
Fujita
Insights into the mechanism of ...
Achromobacter cycloclastes
Inorg. Chem.
46
613-615
2007
-
-
-
-
-
-
-
1
-
1
-
1
-
2
-
-
-
-
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-
1
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2
-
2
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1
1
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1
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1
-
1
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1
-
2
-
-
-
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1
1
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-
-
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-
698456
Ghosh
Spectroscopic, computational, ...
Achromobacter cycloclastes, Marinobacter hydrocarbonoclasticus
J. Am. Chem. Soc.
129
3955-3965
2007
-
-
-
-
-
-
-
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-
2
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5
-
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6
2
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2
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699384
Fujita
Anaerobic purification, charac ...
Achromobacter cycloclastes, Achromobacter cycloclastes IAM1013
J. Inorg. Biochem.
101
1836-1844
2007
-
-
1
-
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1
-
1
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2
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3
-
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1
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1
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2
1
2
1
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1
1
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1
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1
-
1
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2
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1
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1
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2
1
1
-
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-
1
1
-
-
-
-
-
-
-
-
700303
Bingham
-
Magnetic circular dichroism an ...
Paracoccus pantotrophus
Mol. Phys.
105
2169-2176
2007
-
-
-
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1
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1
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1
1
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1
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-
671444
Horn
Nitrous oxide reductase genes ...
Dechloromonas denitrificans, Flavobacterium denitrificans, Pseudomonas sp., Pseudomonas sp. ED3
Appl. Environ. Microbiol.
72
1019-1026
2006
-
-
-
-
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-
-
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-
13
-
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1
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4
-
3
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-
1
-
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4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671455
Sameshima-Saito
Symbiotic Bradyrhizobium japon ...
Bradyrhizobium japonicum, Bradyrhizobium japonicum USDA 110
Appl. Environ. Microbiol.
72
2526-2532
2006
-
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1
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-
-
-
-
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-
-
24
-
-
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-
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2
-
3
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1
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2
-
-
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-
-
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-
-
-
-
-
-
-
-
-
671469
Henry
Quantitative detection of the ...
Achromobacter cycloclastes, Alcaligenes faecalis, Bradyrhizobium japonicum, Ensifer adhaerens, Ensifer adhaerens SN611, Hyphomicrobium denitrificans, Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas denitrificans (nomen rejiciendum) CCUG 2519, Pseudomonas fluorescens, Pseudomonas fluorescens C7R12, Sinorhizobium meliloti, Sinorhizobium meliloti 50
Appl. Environ. Microbiol.
72
5181-5189
2006
-
-
-
-
-
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-
-
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14
-
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-
-
-
12
-
16
-
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12
-
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-
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-
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-
672074
Taubner
Structural studies of Apo NosL ...
Achromobacter cycloclastes
Biochemistry
45
12240-12252
2006
-
-
1
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1
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1
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1
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CuA and CuZ are variants of th ...
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Characterization of the membra ...
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Purification and characterizat ...
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394535
Coyne
Nitrous oxide reduction in nod ...
Rhizobium sp., Rhizobium sp. 8A55
Appl. Environ. Microbiol.
53
1168-1170
1987
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394536
Snyder
Purification and some characte ...
Paracoccus denitrificans
J. Biol. Chem.
262
6515-6525
1987
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394538
Michalski
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Purification and characterizat ...
Rhodobacter sphaeroides
Biochim. Biophys. Acta
872
50-60
1986
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394542
Aida
Temporary low oxygen condition ...
Pseudomonas sp., Pseudomonas sp. G59
Can. J. Microbiol.
32
543-547
1986
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394539
Coyle
Nitrous oxide reductase from d ...
Pseudomonas stutzeri
Eur. J. Biochem.
153
459-467
1985
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394540
McEwan
Nitrous oxide reduction by mem ...
Rhodobacter capsulatus, Rhodobacter sphaeroides, Rhodopseudomonas palustris, Rhodopseudomonas palustris PW5, Rhodospirillum rubrum, Rhodospirillum rubrum S1
J. Bacteriol.
164
823-830
1985
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394537
Kucera
-
Amperometric assay of activity ...
Paracoccus denitrificans
Collect. Czech. Chem. Commun.
49
2709-2712
1984
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394541
Snyder
Nitrous oxide reductase and th ...
Paracoccus denitrificans, Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas stutzeri
Biochem. Biophys. Res. Commun.
119
588-592
1984
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394543
Zumft
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A novel kind of multi-copper p ...
Pseudomonas stutzeri
FEBS Lett.
148
107-112
1982
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394544
Matsubara
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Identification of a copper pro ...
Pseudomonas fluorescens
Arch. Microbiol.
132
322-328
1982
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394545
Kristjansson
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Partial purification and chara ...
Paracoccus denitrificans
Curr. Microbiol.
6
247-251
1981
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394546
Kristjansson
First practical assay for solu ...
Paracoccus denitrificans
J. Biol. Chem.
255
704-707
1980
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