Application | Comment | Organism |
---|---|---|
synthesis | high-yield expression of uricase in Escherichia coli and establishment of an efficient three-step protein purification protocol. The purity of the recombinant protein is more than 98% and the specific activity is 38.4 IU/mg | Cyberlindnera jadinii |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Cyberlindnera jadinii |
Crystallization (Comment) | Organism |
---|---|
to 1.93 A resolution. Space group P212121 with unit cell parameters a 69.16 A, b 139.31 A, c 256.33 A, and alpha =beta =gamma =90° | Cyberlindnera jadinii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 2 mM, complete inactivation | Cyberlindnera jadinii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0337 | - |
Urate | pH 8.0, 37°C | Cyberlindnera jadinii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 2 mM, 170% of initial activity | Cyberlindnera jadinii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34160 | - |
x * 34160, MALDI-TOF | Cyberlindnera jadinii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyberlindnera jadinii | P78609 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
38.4 | - |
recombinant protein, pH 8.0, 37°C | Cyberlindnera jadinii |
Storage Stability | Organism |
---|---|
-20°C, 72 h, no loss of activity | Cyberlindnera jadinii |
4°C, 72 h, no loss of activity | Cyberlindnera jadinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | - |
Cyberlindnera jadinii | 5-hydroxyisourate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34160, MALDI-TOF | Cyberlindnera jadinii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Cyberlindnera jadinii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Cyberlindnera jadinii |